Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RJD38_RS04405 Genome accession   NZ_CP134271
Coordinates   996594..997052 (+) Length   152 a.a.
NCBI ID   WP_065430953.1    Uniprot ID   A0A1B1NRX3
Organism   Vibrio scophthalmi strain 20FBVib0200     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 991594..1002052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD38_RS04390 (RJD38_04390) recA 991936..992976 (-) 1041 WP_005593985.1 recombinase RecA Machinery gene
  RJD38_RS04395 (RJD38_04395) pncC 993191..993679 (-) 489 WP_367971403.1 nicotinamide-nucleotide amidase -
  RJD38_RS04400 (RJD38_04400) mutS 993764..996331 (+) 2568 WP_065545003.1 DNA mismatch repair protein MutS -
  RJD38_RS04405 (RJD38_04405) pilA 996594..997052 (+) 459 WP_065430953.1 pilin Machinery gene
  RJD38_RS04410 (RJD38_04410) pilB 997052..998734 (+) 1683 WP_065545002.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RJD38_RS04415 (RJD38_04415) pilC 998788..1000011 (+) 1224 WP_065545001.1 type II secretion system F family protein Machinery gene
  RJD38_RS04420 (RJD38_04420) pilD 1000117..1000986 (+) 870 WP_065545000.1 prepilin peptidase Machinery gene
  RJD38_RS04425 (RJD38_04425) coaE 1000991..1001599 (+) 609 WP_065430956.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15721.86 Da        Isoelectric Point: 4.6853

>NTDB_id=879550 RJD38_RS04405 WP_065430953.1 996594..997052(+) (pilA) [Vibrio scophthalmi strain 20FBVib0200]
MNNTLTRSQRGFTLIELMVVMAIVAILAVFAVPAYQDYTKKATMAEFPTIAAPVKLAVEMCALEKASSTSTFATNCSTSA
GHVPSAAINNISVTATNVSGAIQVIAQATSDKGPVKSGEEFILSATYSDQGITWAASCTDAGGNAQSAYCPD

Nucleotide


Download         Length: 459 bp        

>NTDB_id=879550 RJD38_RS04405 WP_065430953.1 996594..997052(+) (pilA) [Vibrio scophthalmi strain 20FBVib0200]
ATGAACAATACGTTAACTCGCTCTCAACGCGGTTTCACTCTTATTGAATTAATGGTCGTCATGGCGATTGTCGCTATATT
GGCGGTGTTCGCGGTTCCTGCCTATCAAGACTACACCAAGAAAGCGACCATGGCGGAGTTTCCTACCATCGCCGCCCCAG
TAAAGCTAGCCGTTGAAATGTGCGCACTGGAAAAAGCCTCAAGTACCAGCACGTTTGCCACTAACTGCAGCACTAGTGCA
GGACATGTACCAAGCGCGGCAATCAACAACATCAGTGTTACCGCGACGAATGTCTCTGGCGCAATACAAGTGATCGCTCA
AGCAACAAGCGATAAAGGCCCGGTGAAATCTGGGGAAGAGTTCATTCTCAGCGCGACGTACAGCGATCAGGGCATTACTT
GGGCGGCCAGTTGTACAGACGCCGGTGGCAATGCACAAAGCGCCTACTGTCCAGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1NRX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

52.597

100

0.533
  pilA Haemophilus influenzae Rd KW20

38.095

96.711

0.368
  pilA Haemophilus influenzae 86-028NP

38.732

93.421

0.362
  pilA2 Legionella pneumophila strain ERS1305867

38.194

94.737

0.362