Detailed information NTG0879553

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RJD38_RS04420
• Genome accession: NZ_CP134271
• Coordinates: 1000117..1000986 (+)
• Length: 289 a.a.
• NCBI ID: WP_065545000.1
• Uniprot ID: A0A1C7F5Z7
• Organism: Vibrio scophthalmi strain 20FBVib0200
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 995117..1005986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJD38_RS04405 (RJD38_04405)	pilA	996594..997052 (+)	459	WP_065430953.1	pilin	Machinery gene
RJD38_RS04410 (RJD38_04410)	pilB	997052..998734 (+)	1683	WP_065545002.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RJD38_RS04415 (RJD38_04415)	pilC	998788..1000011 (+)	1224	WP_065545001.1	type II secretion system F family protein	Machinery gene
RJD38_RS04420 (RJD38_04420)	pilD	1000117..1000986 (+)	870	WP_065545000.1	prepilin peptidase	Machinery gene
RJD38_RS04425 (RJD38_04425)	coaE	1000991..1001599 (+)	609	WP_065430956.1	dephospho-CoA kinase	-
RJD38_RS04430 (RJD38_04430)	zapD	1001634..1002374 (+)	741	WP_005593976.1	cell division protein ZapD	-
RJD38_RS04435 (RJD38_04435)	yacG	1002450..1002644 (+)	195	WP_005593974.1	DNA gyrase inhibitor YacG	-
RJD38_RS04440 (RJD38_04440)	rplS	1002849..1003202 (-)	354	WP_005593972.1	50S ribosomal protein L19	-
RJD38_RS04445 (RJD38_04445)	trmD	1003252..1004001 (-)	750	WP_005593971.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
RJD38_RS04450 (RJD38_04450)	rimM	1004029..1004583 (-)	555	WP_005593970.1	ribosome maturation factor RimM	-
RJD38_RS04455 (RJD38_04455)	rpsP	1004608..1004856 (-)	249	WP_005593968.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32382.69 Da
• Isoelectric Point: 7.5693

>NTDB_id=879553 RJD38_RS04420 WP_065545000.1 1000117..1000986(+) (pilD) [Vibrio scophthalmi strain 20FBVib0200]
MELFVYYPWLFPLLATVFGLIVGSFLNVVIYRLPIMMEREWRHDCAESFPEYKIEVPQETFNLSVPRSACPSCHTQIRAI
DNIPVISWLLLKGKCHNCQSKISARYPLIELLTGGLCLLVATTLPASYFTIALLFFTFVLVAATFIDLDTMLLPDSLTLP
LMWSGIALALFGISPVSLQNSVIGAMAGYLCLWSVYWAFKLITKKEGMGYGDFKLLAALGAWLGWQYLPMIILVSSLVGL
VFGLIQLRLQSKGIDKAFPFGPYLAIAGWIAMLWGEQILHGYYRFLLGA

Nucleotide

• Length: 870 bp

>NTDB_id=879553 RJD38_RS04420 WP_065545000.1 1000117..1000986(+) (pilD) [Vibrio scophthalmi strain 20FBVib0200]
ATGGAACTGTTCGTTTATTACCCTTGGCTCTTCCCACTTCTTGCGACGGTGTTTGGTCTTATCGTTGGCAGCTTTCTCAA
TGTGGTCATCTACCGCTTACCGATTATGATGGAGCGCGAGTGGCGTCACGATTGTGCAGAAAGTTTTCCCGAATACAAAA
TTGAAGTTCCACAAGAGACATTTAACCTCAGCGTCCCACGATCAGCTTGCCCGAGTTGCCATACCCAGATTCGCGCCATC
GATAACATTCCTGTTATCAGCTGGCTACTGCTAAAAGGCAAATGCCACAACTGCCAAAGCAAAATCAGTGCTCGCTACCC
TCTGATCGAGTTGCTCACGGGGGGCTTGTGCTTGCTGGTTGCCACCACCTTACCCGCCAGTTACTTCACTATCGCGCTGC
TGTTTTTTACCTTCGTTTTAGTCGCAGCGACGTTTATCGATCTTGATACCATGTTGCTGCCTGATAGCCTGACTCTACCA
CTAATGTGGAGCGGCATTGCCTTAGCCCTGTTTGGCATCAGTCCAGTGAGCTTGCAAAATTCGGTCATTGGCGCCATGGC
CGGCTATTTGTGCCTATGGAGTGTCTACTGGGCTTTCAAACTCATCACCAAAAAAGAAGGTATGGGTTACGGTGATTTTA
AATTGCTTGCCGCGCTAGGCGCCTGGCTTGGCTGGCAGTACCTGCCGATGATCATCTTAGTCTCATCTTTGGTGGGATTG
GTGTTTGGGCTTATTCAATTACGTCTACAGAGCAAAGGCATTGATAAAGCGTTTCCATTTGGTCCTTACTTAGCCATCGC
TGGTTGGATCGCAATGTTATGGGGGGAGCAGATCCTCCATGGCTACTACCGTTTTCTACTAGGAGCGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

3D structure

• AlphaFold DB: A0A1C7F5Z7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	76.736	99.654	0.765
pilD	Vibrio cholerae strain A1552	69.792	99.654	0.696
pilD	Neisseria gonorrhoeae MS11	53.16	93.08	0.495
pilD	Acinetobacter nosocomialis M2	53.008	92.042	0.488
pilD	Acinetobacter baumannii D1279779	52.632	92.042	0.484