Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RJ609_RS19750 Genome accession   NZ_CP134269
Coordinates   4784068..4785795 (+) Length   575 a.a.
NCBI ID   WP_310978545.1    Uniprot ID   -
Organism   Lysobacter capsici strain XL2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4779068..4790795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ609_RS19730 (RJ609_19730) - 4780341..4781501 (-) 1161 WP_310978540.1 glycosyltransferase -
  RJ609_RS19735 (RJ609_19735) - 4781491..4782366 (-) 876 WP_310978541.1 hypothetical protein -
  RJ609_RS19740 (RJ609_19740) - 4782347..4783048 (-) 702 WP_310978543.1 class I SAM-dependent methyltransferase -
  RJ609_RS19745 (RJ609_19745) - 4783039..4783932 (-) 894 WP_310978544.1 glycosyltransferase family 2 protein -
  RJ609_RS19750 (RJ609_19750) pilB 4784068..4785795 (+) 1728 WP_310978545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RJ609_RS19755 (RJ609_19755) pilC 4785799..4787055 (+) 1257 WP_036104584.1 type II secretion system F family protein Machinery gene
  RJ609_RS19760 (RJ609_19760) - 4787077..4787964 (+) 888 WP_036104586.1 A24 family peptidase -
  RJ609_RS19765 (RJ609_19765) coaE 4787968..4788588 (+) 621 WP_310978547.1 dephospho-CoA kinase -
  RJ609_RS19770 (RJ609_19770) - 4788738..4789694 (-) 957 WP_310981870.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62936.43 Da        Isoelectric Point: 6.3668

>NTDB_id=879523 RJ609_RS19750 WP_310978545.1 4784068..4785795(+) (pilB) [Lysobacter capsici strain XL2]
MSTVATANLVGITGIARRLVMDGALDEVKAREAMTAASAERKPLAYYLAEHRLATSAQLAAANSIEFGVPLFDPSTMDPG
QAPIRAISEELIRKHNALPLFKRGNKLFIGLADPTNTKALDEIKFQTNAAVEAILVDEELIRRTLDRWLETSDALGDAVG
DSEGLDNLEIGKDDDLSTTSDTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHPRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDYQKELF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEIASGFKLYEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIRSGVPDLRRSALVKVM
NGVTGLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=879523 RJ609_RS19750 WP_310978545.1 4784068..4785795(+) (pilB) [Lysobacter capsici strain XL2]
ATGTCCACTGTCGCGACCGCCAACTTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGATGGGGCTCTGGACGA
GGTCAAGGCGCGCGAAGCCATGACCGCCGCCAGCGCCGAACGCAAGCCGCTGGCCTATTACCTGGCCGAACATCGCCTGG
CCACTTCGGCCCAGCTCGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACCCGAGCACCATGGACCCGGGC
CAGGCCCCGATCCGGGCGATCAGCGAAGAGCTGATCCGCAAGCACAACGCCTTGCCGCTGTTCAAGCGCGGCAACAAGCT
GTTCATCGGCCTGGCCGATCCGACCAACACCAAGGCCTTGGACGAGATCAAGTTCCAGACCAATGCCGCGGTCGAGGCGA
TCCTGGTCGATGAGGAACTGATACGCCGCACCCTGGACCGCTGGCTGGAGACCTCCGACGCCCTCGGCGACGCGGTCGGC
GACAGCGAGGGTCTGGACAACCTGGAGATCGGCAAGGACGACGACCTCAGCACCACCAGCGACACCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATCCGTCGCGGCGCGTCCGACATCCATT
TCGAACCCTACGAAACCGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCGAACAAG
CTGCATCCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGACG
CATCAAGCTAAACCTTTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGATGGCAGCGCGGCCCGGCTCGGCATCGAAAAGCTCGGCTACGAGGACTACCAGAAGGAATTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAACAACGCAATATCTCAACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATTCGCGACCTCGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCTCCGCAGACCATCGCCCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGT
CGGTCACCCTGGTGATCGCACAGCGTCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAACACGCG
CTGCTCGCCGAGGGGTTCACCCACGAGGAGATCGCGTCGGGTTTCAAGTTGTACGAGCCGGTCGGATGTCCGGACTGTAC
CGAAGGCTACAAGGGACGTACCGGCATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAAGGCG
GCAATGCGATGCAGATCGCCGCCGCGGCGATCCGATCGGGCGTGCCCGATCTGCGCCGCTCCGCGCTGGTCAAGGTGATG
AACGGCGTCACCGGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.185

98.957

0.546
  pilB Acinetobacter baylyi ADP1

55.379

98.609

0.546
  pilB Legionella pneumophila strain ERS1305867

50.613

99.304

0.503
  pilB Vibrio cholerae strain A1552

52.427

89.565

0.47
  pilF Neisseria gonorrhoeae MS11

47.443

98.609

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

49.808

90.783

0.452
  pilB Vibrio campbellii strain DS40M4

51.534

85.043

0.438
  pilF Thermus thermophilus HB27

39.734

91.478

0.363