Detailed information NTG0878750

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RI108_RS10155
• Genome accession: NZ_CP134081
• Coordinates: 2215627..2216790 (+)
• Length: 387 a.a.
• NCBI ID: WP_049859379.1
• Uniprot ID: A0AAJ6MV06
• Organism: Pseudomonas coleopterorum strain DJ13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2210627..2221790

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RI108_RS10135 (RI108_10135)	-	2211526..2212404 (-)	879	WP_310792984.1	Ku protein	-
RI108_RS10140 (RI108_10140)	-	2212657..2212914 (+)	258	WP_049859377.1	DUF1652 domain-containing protein	-
RI108_RS10145 (RI108_10145)	-	2213081..2213356 (-)	276	WP_043189034.1	peptidylprolyl isomerase	-
RI108_RS10150 (RI108_10150)	-	2213399..2215540 (-)	2142	WP_310792985.1	ATP-binding protein	-
RI108_RS10155 (RI108_10155)	pilU	2215627..2216790 (+)	1164	WP_049859379.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RI108_RS10160 (RI108_10160)	-	2217654..2218448 (-)	795	WP_375143215.1	endonuclease/exonuclease/phosphatase family protein	-
RI108_RS10165 (RI108_10165)	-	2218519..2219289 (-)	771	WP_310792986.1	3-hydroxybutyrate dehydrogenase	-
RI108_RS10170 (RI108_10170)	-	2219329..2220906 (-)	1578	WP_310792987.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42878.91 Da
• Isoelectric Point: 6.2941

>NTDB_id=878750 RI108_RS10155 WP_049859379.1 2215627..2216790(+) (pilU) [Pseudomonas coleopterorum strain DJ13]
MEIQALLKLLASQDGSDLYLSTGAPPCAKFNGVLKPLSAEALKPGDIAEIAAGIMDAEQRIDFERELEMNLALSIHGVGR
FRVNIFKQRNEVSIVARNIKLDIPSFEDLKLPRKLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVRTTDGKRRAAVEVMLGTPTIRDFIHRNEFSELKGIMEKSVNLGMQTFDHAL
YELAVEGAISEEEALKNADSVNNLRLRLKLQGEEGVQHTTAPAPPTPAPDRKSADWGLVADDPETPH

Nucleotide

• Length: 1164 bp

>NTDB_id=878750 RI108_RS10155 WP_049859379.1 2215627..2216790(+) (pilU) [Pseudomonas coleopterorum strain DJ13]
ATGGAAATTCAAGCATTGCTCAAGCTCCTGGCCAGTCAGGACGGCTCCGACCTCTACCTGTCCACCGGTGCGCCACCCTG
TGCCAAGTTCAACGGCGTGCTCAAACCCTTGAGCGCCGAGGCGTTGAAGCCCGGCGACATCGCCGAGATCGCGGCCGGCA
TCATGGACGCCGAGCAGCGTATCGATTTCGAGCGCGAGCTGGAAATGAATCTGGCGCTGTCGATCCACGGGGTAGGGCGC
TTTCGCGTCAACATCTTCAAGCAGCGCAACGAAGTGTCCATTGTCGCACGCAACATCAAGCTCGACATTCCCAGCTTCGA
AGACCTCAAGCTGCCGCGCAAGCTGCTCGACGTGATCATGGAAAAACGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTT
CGGGCAAGTCGACGTCGCTGGCAGCGTTGATCGACTACCGCAACCGTAACAGCAGCGGGCATATCATCACCATCGAGGAC
CCGGTGGAATTCATCCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTGGGGGTCGATACGCGCAGTTTCCAGGC
GGCGTTGAAAAACACCCTGCGCCAGGCGCCGGACGTCATCCTGATCGGCGAGATCCGCGACCGTGAAACCATGGAGCATG
CGCTGGCGTTCGCCGACACCGGCCACTTGGCCATTTCAACTCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGACCGCCGTCCGCAGTTGCTCAACGACCTGGGCAACAACCTGCAGGCGTTCGTCTCACAGCG
GCTGGTGCGCACCACCGACGGCAAGCGCCGCGCGGCGGTGGAAGTGATGCTGGGCACCCCGACCATCCGCGATTTCATTC
ACCGCAACGAGTTTTCCGAGCTAAAAGGCATCATGGAGAAATCCGTGAACCTGGGCATGCAGACCTTCGACCATGCGCTC
TACGAGCTGGCAGTGGAAGGGGCCATCAGTGAGGAAGAGGCGTTAAAGAACGCCGATTCGGTGAACAACCTGCGCCTGCG
CTTGAAACTGCAAGGCGAAGAAGGCGTGCAACACACCACCGCACCAGCCCCACCCACACCAGCCCCCGACCGCAAAAGCG
CAGATTGGGGCCTGGTGGCGGATGACCCAGAAACCCCGCACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.54	0.543
pilU	Acinetobacter baylyi ADP1	53.521	91.731	0.491
pilU	Vibrio cholerae strain A1552	51.429	90.439	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.628	97.158	0.385
pilT	Pseudomonas stutzeri DSM 10701	41.787	89.664	0.375
pilT	Pseudomonas aeruginosa PAK	40.988	88.889	0.364