NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RHS40_RS06130	Genome accession	NZ_CP133836
Coordinates	1315043..1315477 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain sample-B
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1310043..1320477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHS40_RS06100 (RHS40_06100)	-	1310610..1310879 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RHS40_RS06105 (RHS40_06105)	yacG	1310879..1311082 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RHS40_RS06110 (RHS40_06110)	coaE	1311093..1311713 (-)	621	WP_005751078.1	dephospho-CoA kinase	-
RHS40_RS06115 (RHS40_06115)	-	1311728..1312450 (-)	723	WP_005727302.1	A24 family peptidase	-
RHS40_RS06120 (RHS40_06120)	-	1312450..1313661 (-)	1212	WP_005751077.1	type II secretion system F family protein	-
RHS40_RS06125 (RHS40_06125)	pilB	1313661..1315043 (-)	1383	WP_005751075.1	GspE/PulE family protein	Machinery gene
RHS40_RS06130 (RHS40_06130)	pilA	1315043..1315477 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RHS40_RS06135 (RHS40_06135)	ampD	1315637..1316203 (+)	567	WP_005751074.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RHS40_RS06140 (RHS40_06140)	rppH	1316789..1317385 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RHS40_RS06145 (RHS40_06145)	-	1317388..1318182 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RHS40_RS06150 (RHS40_06150)	lgt	1318192..1318995 (+)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
RHS40_RS06155 (RHS40_06155)	-	1319001..1319852 (+)	852	WP_005751072.1	thymidylate synthase	-
RHS40_RS06160 (RHS40_06160)	tadA	1319867..1320367 (+)	501	WP_005751071.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=877065 RHS40_RS06130 WP_005741096.1 1315043..1315477(-) (pilA) [Pasteurella multocida strain sample-B]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=877065 RHS40_RS06130 WP_005741096.1 1315043..1315477(-) (pilA) [Pasteurella multocida strain sample-B]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC

AATCTGACATTTTTCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0877065