NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RHO09_RS05750	Genome accession	NZ_CP133810
Coordinates	1255306..1255740 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain W22-3735.1
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1250306..1260740

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHO09_RS05720 (RHO09_05720)	-	1250870..1251139 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RHO09_RS05725 (RHO09_05725)	yacG	1251139..1251342 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RHO09_RS05730 (RHO09_05730)	coaE	1251353..1251973 (-)	621	WP_250191472.1	dephospho-CoA kinase	-
RHO09_RS05735 (RHO09_05735)	-	1251988..1252710 (-)	723	WP_310850445.1	A24 family peptidase	-
RHO09_RS05740 (RHO09_05740)	-	1252710..1253921 (-)	1212	WP_005753354.1	type II secretion system F family protein	-
RHO09_RS05745 (RHO09_05745)	pilB	1253921..1255306 (-)	1386	WP_046333442.1	GspE/PulE family protein	Machinery gene
RHO09_RS05750 (RHO09_05750)	pilA	1255306..1255740 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RHO09_RS05755 (RHO09_05755)	ampD	1255900..1256466 (+)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RHO09_RS05760 (RHO09_05760)	rppH	1257052..1257648 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RHO09_RS05765 (RHO09_05765)	-	1257651..1258445 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RHO09_RS05770 (RHO09_05770)	lgt	1258455..1259267 (+)	813	WP_156732338.1	prolipoprotein diacylglyceryl transferase	-
RHO09_RS05775 (RHO09_05775)	-	1259264..1260115 (+)	852	WP_310850451.1	thymidylate synthase	-
RHO09_RS05780 (RHO09_05780)	tadA	1260130..1260630 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=876998 RHO09_RS05750 WP_005741096.1 1255306..1255740(-) (pilA) [Pasteurella multocida strain W22-3735.1]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=876998 RHO09_RS05750 WP_005741096.1 1255306..1255740(-) (pilA) [Pasteurella multocida strain W22-3735.1]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0876998