Detailed information NTG0876738

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RHM59_RS06185
• Genome accession: NZ_CP133783
• Coordinates: 1379847..1381025 (-)
• Length: 392 a.a.
• NCBI ID: WP_322169497.1
• Uniprot ID: -
• Organism: Pseudomonas sp. MH10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1374847..1386025

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHM59_RS06170 (RHM59_06170)	-	1375682..1377073 (+)	1392	WP_322286201.1	GntP family permease	-
RHM59_RS06175 (RHM59_06175)	-	1377084..1377866 (+)	783	WP_322286202.1	3-hydroxybutyrate dehydrogenase	-
RHM59_RS06180 (RHM59_06180)	-	1377879..1379834 (+)	1956	WP_322286203.1	acetoacetate--CoA ligase	-
RHM59_RS06185 (RHM59_06185)	pilU	1379847..1381025 (-)	1179	WP_322169497.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RHM59_RS06190 (RHM59_06190)	-	1381232..1381507 (+)	276	WP_322169494.1	peptidylprolyl isomerase	-
RHM59_RS06195 (RHM59_06195)	-	1381802..1383982 (-)	2181	WP_322286204.1	lipase family protein	-
RHM59_RS06200 (RHM59_06200)	-	1383992..1384990 (-)	999	WP_322169487.1	hypothetical protein	-
RHM59_RS06205 (RHM59_06205)	-	1385138..1385356 (-)	219	WP_322286205.1	hypothetical protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43493.99 Da
• Isoelectric Point: 6.3247

>NTDB_id=876738 RHM59_RS06185 WP_322169497.1 1379847..1381025(-) (pilU) [Pseudomonas sp. MH10]
MDFQALLKILASQDGSDLFLSTGAPPCAKFNGVLKPLSTEALKPGDVASVADSIMDAEQRLEFERELEMNLAVSLAGIGR
FRINIFKQRNEVSIVARNIKLEIPRFEDLKLPPVLLEVVMEKRGLVLVIGATGSGKSTSLAAMIDYRNRNSGGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRQQLLHDLGNNLKAFVSQRLVKTLDGKRRAAVEVMLGTPTILDFIQRNELTELKGIMEKSTNLGMQTFDGAL
FDLVVEGAISEEEALKNADSKNNVRLRLKLFREKGLPTQITSAPAAPEPAVESTMANWGLVDEMEEPSRFKP

Nucleotide

• Length: 1179 bp

>NTDB_id=876738 RHM59_RS06185 WP_322169497.1 1379847..1381025(-) (pilU) [Pseudomonas sp. MH10]
ATGGATTTCCAAGCGCTGTTGAAAATTCTGGCCAGCCAGGACGGATCCGACCTTTTCTTGTCCACCGGCGCGCCGCCCTG
CGCAAAGTTCAATGGGGTGCTTAAGCCGCTGAGCACCGAGGCGTTGAAGCCCGGCGACGTGGCGAGCGTTGCCGACAGCA
TCATGGATGCCGAACAGCGGCTGGAATTCGAACGCGAGCTGGAGATGAACCTGGCGGTGTCACTGGCAGGCATCGGTCGA
TTTCGGATCAATATTTTCAAGCAGCGCAACGAAGTCTCGATAGTGGCGCGCAACATCAAGCTGGAGATACCGCGTTTCGA
GGATTTGAAGTTACCGCCGGTGCTGCTCGAAGTGGTCATGGAAAAGCGCGGCTTGGTGCTGGTCATTGGCGCCACCGGTT
CGGGTAAGTCGACGTCGCTGGCGGCGATGATCGATTACCGAAATCGCAACAGTGGCGGCCACATCATCACCATTGAAGAC
CCGGTGGAGTATATCCATAGGCACAAGAAGTCGATCATCAATCAGCGTGAAGTGGGCGTAGACACCCGCAGTTTTCACGC
CGCACTGAAGAACACCTTACGTCAGGCGCCGGATGTGATTTTGATCGGCGAGATTCGTGACCGCGAAACCATGGAACACG
CGTTGGCGTTTGCCGACACCGGCCACTTGGCTATTTCGACCTTGCACGCCAACAACGCCAACCAGGCCCTGGACCGGATC
ATTAACTTTTTCCCGGAAGACCGGCGCCAGCAGTTGCTGCATGACTTGGGCAACAACTTGAAGGCATTTGTGTCCCAGCG
GCTGGTCAAGACTCTGGACGGTAAACGCCGCGCGGCGGTGGAAGTCATGCTGGGCACACCAACCATTCTTGATTTTATTC
AGCGCAATGAACTGACTGAACTCAAGGGCATCATGGAGAAGTCCACCAACCTTGGGATGCAGACCTTCGACGGCGCGCTG
TTTGACTTAGTGGTCGAGGGCGCGATCAGTGAAGAGGAAGCCCTGAAGAACGCCGACTCCAAGAACAACGTACGTCTGCG
CCTCAAGCTGTTCCGCGAGAAAGGCTTGCCGACTCAAATCACTTCCGCACCAGCCGCCCCCGAGCCCGCCGTAGAGTCGA
CCATGGCGAACTGGGGGCTGGTGGACGAGATGGAAGAACCATCTCGGTTCAAGCCTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.91	90.306	0.523
pilU	Acinetobacter baylyi ADP1	54.596	91.582	0.5
pilU	Vibrio cholerae strain A1552	51.558	90.051	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.467	95.663	0.378
pilT	Legionella pneumophila strain Lp02	41.52	87.245	0.362
pilT	Legionella pneumophila strain ERS1305867	41.52	87.245	0.362