Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHM61_RS08910 Genome accession   NZ_CP133779
Coordinates   1938937..1940664 (-) Length   575 a.a.
NCBI ID   WP_322250763.1    Uniprot ID   A0AAU0SB23
Organism   Undibacterium sp. CCC3.4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1933937..1945664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM61_RS08885 (RHM61_08885) - 1935179..1935355 (-) 177 WP_322250758.1 DNA gyrase inhibitor YacG -
  RHM61_RS08890 (RHM61_08890) zapD 1935358..1936113 (-) 756 WP_322250759.1 cell division protein ZapD -
  RHM61_RS08895 (RHM61_08895) coaE 1936220..1936837 (-) 618 WP_322250760.1 dephospho-CoA kinase -
  RHM61_RS08900 (RHM61_08900) pilD 1936845..1937696 (-) 852 WP_322250761.1 A24 family peptidase Machinery gene
  RHM61_RS08905 (RHM61_08905) pilC 1937696..1938922 (-) 1227 WP_322250762.1 type II secretion system F family protein Machinery gene
  RHM61_RS08910 (RHM61_08910) pilB 1938937..1940664 (-) 1728 WP_322250763.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM61_RS08915 (RHM61_08915) ispB 1940854..1941783 (-) 930 WP_322251074.1 octaprenyl diphosphate synthase -
  RHM61_RS08920 (RHM61_08920) rplU 1942119..1942430 (+) 312 WP_322250764.1 50S ribosomal protein L21 -
  RHM61_RS08925 (RHM61_08925) rpmA 1942463..1942720 (+) 258 WP_322250765.1 50S ribosomal protein L27 -
  RHM61_RS08930 (RHM61_08930) obgE 1942891..1944003 (+) 1113 WP_322250766.1 GTPase ObgE -
  RHM61_RS08935 (RHM61_08935) - 1944950..1945171 (-) 222 WP_322250767.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62857.49 Da        Isoelectric Point: 5.0450

>NTDB_id=876696 RHM61_RS08910 WP_322250763.1 1938937..1940664(-) (pilB) [Undibacterium sp. CCC3.4]
MSAAPPNSAPPSLTAGLARALLQANLISLVQIDVITKKATTDKTAFIDGLLQSGIINARELASFCSETFGYPLLDLSIIN
EGLIPEKLVDQRLMQLQRLVPISKKGNKLTLAVSDPTNSSALDQVKFQTNLAIEIIIVQHDALVKLLEKIGKTSEQSLND
LAGDDFDIEFREEDLGQAAADAQATEVDDAPIVKFLQKMLIDAINMGASDLHFEPFEKFYRIRFRVDGELREIAQPPLAI
KDKLSSRIKVISKLDISEKRIPQDGRMKLHLNANKSIDFRVSTLPTLFGEKIVMRILDGSQAQMGIDALGYDPDQKEILM
NAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNVNDKAGLSFAAALKSFLRQDPDVI
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKRPAEVLDEVL
LQAGFKEEELDGSWKPYEPVGCERCNGGGYKGRVGIYQLMPITSAIERIILSNGTAMDIEEQAQREGVRNLRQSGLMKVK
QGMTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=876696 RHM61_RS08910 WP_322250763.1 1938937..1940664(-) (pilB) [Undibacterium sp. CCC3.4]
ATGTCCGCCGCCCCACCAAACTCCGCACCACCCTCGTTGACCGCAGGATTAGCCCGGGCCTTGCTGCAAGCTAATTTGAT
CAGCTTGGTACAAATCGATGTGATCACTAAAAAGGCAACGACGGATAAAACCGCTTTCATTGATGGTCTGCTGCAAAGCG
GCATCATCAATGCGCGTGAATTAGCCAGTTTTTGTTCCGAAACCTTCGGTTATCCGCTGCTCGATCTGAGCATCATCAAT
GAAGGTCTGATTCCGGAAAAACTGGTCGATCAACGTCTGATGCAATTACAGCGTTTGGTACCAATTTCAAAAAAAGGCAA
TAAGCTCACGCTGGCCGTTTCTGACCCGACCAATAGCTCCGCGCTCGATCAAGTCAAATTCCAGACCAATCTTGCTATCG
AGATCATCATTGTGCAGCATGATGCGCTGGTCAAGCTGTTGGAAAAAATCGGTAAAACCTCGGAACAAAGTCTCAACGAC
TTGGCCGGTGACGATTTCGATATCGAATTTCGCGAAGAGGACCTCGGGCAAGCTGCTGCCGACGCCCAAGCGACCGAAGT
CGACGATGCCCCGATCGTGAAATTTCTGCAGAAAATGCTGATCGACGCTATCAATATGGGTGCCTCCGATTTGCATTTCG
AACCGTTCGAAAAGTTTTACCGCATCCGCTTCCGCGTCGACGGTGAGTTGCGCGAAATTGCCCAACCACCATTGGCAATC
AAAGACAAGCTGTCGTCGCGCATCAAGGTGATCTCTAAGCTCGATATTTCAGAAAAGCGCATCCCACAAGATGGCCGGAT
GAAATTGCATCTCAACGCCAATAAGTCCATCGATTTCCGCGTCAGCACGCTGCCTACGCTGTTCGGCGAAAAAATCGTGA
TGCGGATTCTCGATGGCTCGCAAGCGCAGATGGGTATCGATGCGCTCGGCTACGACCCGGATCAAAAAGAAATTCTGATG
AATGCGATTCAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCTACCGGCTCGGGCAAGACGGTGTCGCTATATACCTG
TCTCAACGTCATCAATAAGCCGGGTATCAATATCTCGACGGCCGAAGATCCGGCCGAGATCAATTTACCGGGCGTCAACC
AAGTGAATGTCAATGACAAGGCCGGGCTGAGCTTTGCCGCCGCACTGAAATCATTTTTGCGGCAGGATCCGGATGTCATC
ATGGTGGGTGAAATTCGCGACTTGGAAACTGCCGACATCGCCATCAAGGCGGCGCAAACCGGCCATATGGTGTTCTCTAC
CCTGCATACCAATGATGCCCCGTCGACCCTGACGCGGCTGATGAATATGGGCGTGGCACCGTTCAATATCGCCTCATCGG
TGATTTTGATCACGGCGCAGCGGCTGGCACGCCGTCTGTGTACCTGCAAGCGTCCGGCCGAGGTACTCGATGAAGTACTG
CTGCAAGCCGGTTTTAAAGAAGAAGAACTCGACGGCAGTTGGAAGCCATATGAACCGGTTGGCTGCGAGCGCTGCAACGG
CGGCGGCTACAAAGGCCGCGTCGGCATTTATCAACTCATGCCCATTACCAGCGCAATCGAACGCATCATTCTCTCGAATG
GCACGGCGATGGATATTGAAGAGCAAGCGCAGCGCGAAGGCGTGCGCAATCTGCGCCAGTCTGGCTTGATGAAGGTTAAA
CAAGGCATGACCAGTTTGGAAGAAGTATTGGGATGTACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.509

99.13

0.53
  pilB Acinetobacter baylyi ADP1

53.158

99.13

0.527
  pilF Neisseria gonorrhoeae MS11

52.827

98.435

0.52
  pilB Legionella pneumophila strain ERS1305867

49.553

97.217

0.482
  pilB Vibrio cholerae strain A1552

47.972

98.609

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

45.724

99.652

0.456
  pilB Vibrio campbellii strain DS40M4

45.053

98.435

0.443
  pilF Thermus thermophilus HB27

40.147

94.435

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.312

96

0.377