Detailed information NTG0876617

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RHM64_RS09915
• Genome accession: NZ_CP133776
• Coordinates: 2221321..2222499 (+)
• Length: 392 a.a.
• NCBI ID: WP_322169497.1
• Uniprot ID: -
• Organism: Pseudomonas sp. AH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2216321..2227499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHM64_RS09900 (RHM64_09900)	-	2217357..2218355 (+)	999	WP_322169487.1	hypothetical protein	-
RHM64_RS09905 (RHM64_09905)	-	2218365..2220518 (+)	2154	WP_322169490.1	lipase family protein	-
RHM64_RS09910 (RHM64_09910)	-	2220839..2221114 (-)	276	WP_322169494.1	peptidylprolyl isomerase	-
RHM64_RS09915 (RHM64_09915)	pilU	2221321..2222499 (+)	1179	WP_322169497.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RHM64_RS09920 (RHM64_09920)	-	2222512..2224467 (-)	1956	WP_322169501.1	acetoacetate--CoA ligase	-
RHM64_RS09925 (RHM64_09925)	-	2224480..2225262 (-)	783	WP_322169505.1	3-hydroxybutyrate dehydrogenase	-
RHM64_RS09930 (RHM64_09930)	-	2225273..2226664 (-)	1392	WP_322169507.1	GntP family permease	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43493.99 Da
• Isoelectric Point: 6.3247

>NTDB_id=876617 RHM64_RS09915 WP_322169497.1 2221321..2222499(+) (pilU) [Pseudomonas sp. AH2]
MDFQALLKILASQDGSDLFLSTGAPPCAKFNGVLKPLSTEALKPGDVASVADSIMDAEQRLEFERELEMNLAVSLAGIGR
FRINIFKQRNEVSIVARNIKLEIPRFEDLKLPPVLLEVVMEKRGLVLVIGATGSGKSTSLAAMIDYRNRNSGGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRQQLLHDLGNNLKAFVSQRLVKTLDGKRRAAVEVMLGTPTILDFIQRNELTELKGIMEKSTNLGMQTFDGAL
FDLVVEGAISEEEALKNADSKNNVRLRLKLFREKGLPTQITSAPAAPEPAVESTMANWGLVDEMEEPSRFKP

Nucleotide

• Length: 1179 bp

>NTDB_id=876617 RHM64_RS09915 WP_322169497.1 2221321..2222499(+) (pilU) [Pseudomonas sp. AH2]
ATGGATTTCCAAGCGCTGTTGAAAATTCTGGCCAGCCAGGACGGATCCGACCTTTTCTTGTCCACCGGCGCGCCGCCCTG
CGCAAAGTTCAATGGGGTGCTTAAGCCGCTGAGCACCGAGGCGTTGAAGCCCGGCGACGTGGCGAGCGTTGCCGACAGCA
TCATGGATGCCGAACAGCGGCTGGAATTCGAACGCGAGCTGGAGATGAACCTGGCGGTGTCACTGGCAGGCATCGGTCGA
TTTCGGATCAATATTTTCAAGCAGCGCAACGAAGTCTCGATAGTGGCGCGCAACATCAAGCTGGAGATACCGCGTTTCGA
GGATTTGAAGTTACCGCCGGTGCTGCTCGAAGTGGTCATGGAAAAGCGTGGCTTGGTGCTGGTCATTGGCGCCACCGGTT
CGGGTAAGTCGACGTCGCTGGCGGCGATGATCGATTACCGAAATCGCAACAGTGGCGGCCACATCATCACCATTGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAATCAGCGTGAAGTGGGCGTAGACACCCGCAGTTTTCACGC
CGCACTGAAGAACACCTTACGTCAGGCGCCGGATGTGATTTTGATCGGCGAGATTCGTGACCGCGAAACCATGGAACACG
CGTTGGCGTTTGCCGACACCGGCCACTTGGCTATTTCGACCTTGCACGCCAACAACGCCAACCAGGCCCTGGACCGGATC
ATTAACTTTTTCCCGGAAGACCGGCGCCAGCAGTTGCTGCATGACTTGGGCAACAACTTGAAGGCATTTGTGTCCCAGCG
GCTGGTCAAGACTCTGGACGGCAAACGCCGCGCGGCGGTGGAAGTCATGCTGGGCACGCCAACCATTCTTGATTTTATTC
AGCGCAATGAACTGACTGAACTCAAGGGCATCATGGAGAAGTCCACCAACCTTGGGATGCAGACCTTCGACGGCGCGCTG
TTTGACTTGGTGGTCGAGGGCGCGATCAGTGAAGAGGAAGCCCTGAAGAACGCCGACTCCAAGAACAACGTACGTCTGCG
CCTCAAGCTGTTCCGCGAGAAAGGCTTGCCGACTCAAATCACTTCCGCACCAGCCGCCCCCGAGCCCGCCGTAGAGTCGA
CCATGGCGAACTGGGGGCTGGTGGACGAGATGGAAGAGCCATCTCGGTTCAAGCCTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.91	90.306	0.523
pilU	Acinetobacter baylyi ADP1	54.596	91.582	0.5
pilU	Vibrio cholerae strain A1552	51.558	90.051	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.467	95.663	0.378
pilT	Legionella pneumophila strain Lp02	41.52	87.245	0.362
pilT	Legionella pneumophila strain ERS1305867	41.52	87.245	0.362