Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RE654_RS00325 Genome accession   NZ_CP133757
Coordinates   62170..63279 (-) Length   369 a.a.
NCBI ID   WP_041214461.1    Uniprot ID   A0A839D3X0
Organism   Aeromonas caviae strain LZSFT54     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 57170..68279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE654_RS00300 (RE654_00300) - 57304..57531 (+) 228 WP_039039956.1 hypothetical protein -
  RE654_RS00305 (RE654_00305) brnQ 57621..58928 (-) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -
  RE654_RS00310 (RE654_00310) - 59118..59828 (-) 711 WP_039039957.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  RE654_RS00315 (RE654_00315) srmB 59956..61176 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  RE654_RS00320 (RE654_00320) yaaA 61364..62137 (-) 774 WP_309563247.1 peroxide stress protein YaaA -
  RE654_RS00325 (RE654_00325) pilU 62170..63279 (-) 1110 WP_041214461.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RE654_RS00330 (RE654_00330) pilT 63303..64337 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  RE654_RS00335 (RE654_00335) - 64377..65078 (+) 702 WP_309563248.1 YggS family pyridoxal phosphate-dependent enzyme -
  RE654_RS00340 (RE654_00340) proC 65205..66029 (+) 825 WP_309563249.1 pyrroline-5-carboxylate reductase -
  RE654_RS00345 (RE654_00345) - 66053..66604 (+) 552 WP_042014922.1 YggT family protein -
  RE654_RS00350 (RE654_00350) yggU 66604..66903 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  RE654_RS00355 (RE654_00355) - 66922..67341 (+) 420 WP_049636470.1 DUF4426 domain-containing protein -
  RE654_RS00360 (RE654_00360) - 67417..68247 (-) 831 WP_309563250.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40856.99 Da        Isoelectric Point: 6.3155

>NTDB_id=876360 RE654_RS00325 WP_041214461.1 62170..63279(-) (pilU) [Aeromonas caviae strain LZSFT54]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=876360 RE654_RS00325 WP_041214461.1 62170..63279(-) (pilU) [Aeromonas caviae strain LZSFT54]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839D3X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

91.057

0.393
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379