NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RFF10_RS05895	Genome accession	NZ_CP133636
Coordinates	1278147..1278581 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain Past29
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1273147..1283581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFF10_RS05865 (RFF10_05865)	-	1273711..1273980 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RFF10_RS05870 (RFF10_05870)	yacG	1273980..1274183 (-)	204	WP_046333438.1	DNA gyrase inhibitor YacG	-
RFF10_RS05875 (RFF10_05875)	coaE	1274194..1274814 (-)	621	WP_046333439.1	dephospho-CoA kinase	-
RFF10_RS05880 (RFF10_05880)	-	1274829..1275551 (-)	723	WP_046333440.1	prepilin peptidase	-
RFF10_RS05885 (RFF10_05885)	-	1275551..1276762 (-)	1212	WP_046333441.1	type II secretion system F family protein	-
RFF10_RS05890 (RFF10_05890)	pilB	1276762..1278147 (-)	1386	WP_046333442.1	GspE/PulE family protein	Machinery gene
RFF10_RS05895 (RFF10_05895)	pilA	1278147..1278581 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RFF10_RS05900 (RFF10_05900)	ampD	1278741..1279307 (+)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RFF10_RS05905 (RFF10_05905)	rppH	1279893..1280489 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RFF10_RS05910 (RFF10_05910)	-	1280492..1281286 (+)	795	WP_046333443.1	sulfite exporter TauE/SafE family protein	-
RFF10_RS05915 (RFF10_05915)	lgt	1281296..1282099 (+)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
RFF10_RS05920 (RFF10_05920)	-	1282105..1282956 (+)	852	WP_005751072.1	thymidylate synthase	-
RFF10_RS05925 (RFF10_05925)	tadA	1282971..1283471 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=875372 RFF10_RS05895 WP_005741096.1 1278147..1278581(-) (pilA) [Pasteurella multocida strain Past29]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=875372 RFF10_RS05895 WP_005741096.1 1278147..1278581(-) (pilA) [Pasteurella multocida strain Past29]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC

CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0875372