Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RFF39_RS06280 Genome accession   NZ_CP133634
Coordinates   1327114..1328499 (-) Length   461 a.a.
NCBI ID   WP_323997285.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1322114..1333499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF39_RS06250 (RFF39_06250) - 1322241..1323614 (-) 1374 WP_170385405.1 alanine/glycine:cation symporter family protein -
  RFF39_RS06255 (RFF39_06255) - 1324063..1324332 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF39_RS06260 (RFF39_06260) yacG 1324332..1324535 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RFF39_RS06265 (RFF39_06265) coaE 1324546..1325166 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  RFF39_RS06270 (RFF39_06270) - 1325181..1325903 (-) 723 WP_279895731.1 A24 family peptidase -
  RFF39_RS06275 (RFF39_06275) - 1325903..1327114 (-) 1212 WP_151249092.1 type II secretion system F family protein -
  RFF39_RS06280 (RFF39_06280) pilB 1327114..1328499 (-) 1386 WP_323997285.1 GspE/PulE family protein Machinery gene
  RFF39_RS06285 (RFF39_06285) pilA 1328499..1328933 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  RFF39_RS06290 (RFF39_06290) ampD 1329093..1329659 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF39_RS06295 (RFF39_06295) rppH 1330245..1330841 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RFF39_RS06300 (RFF39_06300) - 1330844..1331638 (+) 795 WP_046333443.1 sulfite exporter TauE/SafE family protein -
  RFF39_RS06305 (RFF39_06305) lgt 1331648..1332451 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF39_RS06310 (RFF39_06310) - 1332457..1333308 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52104.74 Da        Isoelectric Point: 6.3696

>NTDB_id=875349 RFF39_RS06280 WP_323997285.1 1327114..1328499(-) (pilB) [Pasteurella multocida strain Past33]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFESALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=875349 RFF39_RS06280 WP_323997285.1 1327114..1328499(-) (pilB) [Pasteurella multocida strain Past33]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAACTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAGGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAAAGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAACCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.433

98.265

0.672
  pilB Haemophilus influenzae Rd KW20

67.77

98.265

0.666
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395