Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RFF39_RS06285 Genome accession   NZ_CP133634
Coordinates   1328499..1328933 (-) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida strain Past33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1323499..1333933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF39_RS06255 (RFF39_06255) - 1324063..1324332 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF39_RS06260 (RFF39_06260) yacG 1324332..1324535 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RFF39_RS06265 (RFF39_06265) coaE 1324546..1325166 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  RFF39_RS06270 (RFF39_06270) - 1325181..1325903 (-) 723 WP_279895731.1 A24 family peptidase -
  RFF39_RS06275 (RFF39_06275) - 1325903..1327114 (-) 1212 WP_151249092.1 type II secretion system F family protein -
  RFF39_RS06280 (RFF39_06280) pilB 1327114..1328499 (-) 1386 WP_323997285.1 GspE/PulE family protein Machinery gene
  RFF39_RS06285 (RFF39_06285) pilA 1328499..1328933 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  RFF39_RS06290 (RFF39_06290) ampD 1329093..1329659 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF39_RS06295 (RFF39_06295) rppH 1330245..1330841 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RFF39_RS06300 (RFF39_06300) - 1330844..1331638 (+) 795 WP_046333443.1 sulfite exporter TauE/SafE family protein -
  RFF39_RS06305 (RFF39_06305) lgt 1331648..1332451 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF39_RS06310 (RFF39_06310) - 1332457..1333308 (+) 852 WP_005751072.1 thymidylate synthase -
  RFF39_RS06315 (RFF39_06315) tadA 1333323..1333823 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=875350 RFF39_RS06285 WP_061406215.1 1328499..1328933(-) (pilA) [Pasteurella multocida strain Past33]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=875350 RFF39_RS06285 WP_061406215.1 1328499..1328933(-) (pilA) [Pasteurella multocida strain Past33]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361