Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RFF30_RS05830 Genome accession   NZ_CP133632
Coordinates   1269506..1270891 (-) Length   461 a.a.
NCBI ID   WP_324006930.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1621     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1264506..1275891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF30_RS05800 (RFF30_05800) - 1264633..1266006 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  RFF30_RS05805 (RFF30_05805) - 1266455..1266724 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF30_RS05810 (RFF30_05810) yacG 1266724..1266927 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RFF30_RS05815 (RFF30_05815) coaE 1266938..1267558 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  RFF30_RS05820 (RFF30_05820) - 1267573..1268295 (-) 723 WP_324006929.1 A24 family peptidase -
  RFF30_RS05825 (RFF30_05825) - 1268295..1269506 (-) 1212 WP_324007555.1 type II secretion system F family protein -
  RFF30_RS05830 (RFF30_05830) pilB 1269506..1270891 (-) 1386 WP_324006930.1 GspE/PulE family protein Machinery gene
  RFF30_RS05835 (RFF30_05835) pilA 1270891..1271325 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  RFF30_RS05840 (RFF30_05840) ampD 1271485..1272051 (+) 567 WP_170385414.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF30_RS05845 (RFF30_05845) rppH 1272637..1273233 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RFF30_RS05850 (RFF30_05850) - 1273236..1274030 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RFF30_RS05855 (RFF30_05855) lgt 1274040..1274843 (+) 804 WP_195188378.1 prolipoprotein diacylglyceryl transferase -
  RFF30_RS05860 (RFF30_05860) - 1274849..1275700 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52161.85 Da        Isoelectric Point: 6.5002

>NTDB_id=875304 RFF30_RS05830 WP_324006930.1 1269506..1270891(-) (pilB) [Pasteurella multocida strain Pm1621]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLMNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFGELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=875304 RFF30_RS05830 WP_324006930.1 1269506..1270891(-) (pilB) [Pasteurella multocida strain Pm1621]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGATATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAACTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAATGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTTCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAGTTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGGTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.265

0.666
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.212

100

0.41
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.085

100

0.408
  pilB Vibrio cholerae strain A1552

38.066

100

0.401
  pilB Legionella pneumophila strain ERS1305867

37.014

100

0.393