Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RFF30_RS05835 Genome accession   NZ_CP133632
Coordinates   1270891..1271325 (-) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida strain Pm1621     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1265891..1276325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF30_RS05805 (RFF30_05805) - 1266455..1266724 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF30_RS05810 (RFF30_05810) yacG 1266724..1266927 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RFF30_RS05815 (RFF30_05815) coaE 1266938..1267558 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  RFF30_RS05820 (RFF30_05820) - 1267573..1268295 (-) 723 WP_324006929.1 A24 family peptidase -
  RFF30_RS05825 (RFF30_05825) - 1268295..1269506 (-) 1212 WP_324007555.1 type II secretion system F family protein -
  RFF30_RS05830 (RFF30_05830) pilB 1269506..1270891 (-) 1386 WP_324006930.1 GspE/PulE family protein Machinery gene
  RFF30_RS05835 (RFF30_05835) pilA 1270891..1271325 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  RFF30_RS05840 (RFF30_05840) ampD 1271485..1272051 (+) 567 WP_170385414.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF30_RS05845 (RFF30_05845) rppH 1272637..1273233 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RFF30_RS05850 (RFF30_05850) - 1273236..1274030 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RFF30_RS05855 (RFF30_05855) lgt 1274040..1274843 (+) 804 WP_195188378.1 prolipoprotein diacylglyceryl transferase -
  RFF30_RS05860 (RFF30_05860) - 1274849..1275700 (+) 852 WP_005751072.1 thymidylate synthase -
  RFF30_RS05865 (RFF30_05865) tadA 1275715..1276215 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=875305 RFF30_RS05835 WP_061406215.1 1270891..1271325(-) (pilA) [Pasteurella multocida strain Pm1621]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=875305 RFF30_RS05835 WP_061406215.1 1270891..1271325(-) (pilA) [Pasteurella multocida strain Pm1621]
ATGAAAAAAGCTATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTCTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361