Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RDV53_RS06550 Genome accession   NZ_CP133470
Coordinates   1326188..1326631 (-) Length   147 a.a.
NCBI ID   WP_005697917.1    Uniprot ID   A0A369YN52
Organism   Haemophilus parainfluenzae ATCC 33392 strain DSM 8978     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1321188..1331631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV53_RS06520 (RDV53_06520) - 1321712..1321993 (-) 282 WP_032822874.1 GNAT family N-acetyltransferase -
  RDV53_RS06525 (RDV53_06525) yacG 1321990..1322196 (-) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  RDV53_RS06530 (RDV53_06530) coaE 1322186..1322809 (-) 624 WP_005695506.1 dephospho-CoA kinase -
  RDV53_RS06535 (RDV53_06535) pilD 1322896..1323573 (-) 678 WP_005695507.1 A24 family peptidase Machinery gene
  RDV53_RS06540 (RDV53_06540) pilC 1323570..1324793 (-) 1224 WP_005695508.1 type II secretion system F family protein Machinery gene
  RDV53_RS06545 (RDV53_06545) pilB 1324802..1326181 (-) 1380 WP_005695509.1 GspE/PulE family protein Machinery gene
  RDV53_RS06550 (RDV53_06550) pilA 1326188..1326631 (-) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  RDV53_RS06555 (RDV53_06555) ampD 1326774..1327337 (+) 564 WP_005695512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RDV53_RS06560 (RDV53_06560) rppH 1327822..1328415 (+) 594 WP_005695513.1 RNA pyrophosphohydrolase -
  RDV53_RS06565 (RDV53_06565) - 1328415..1329209 (+) 795 WP_005695514.1 sulfite exporter TauE/SafE family protein -
  RDV53_RS06570 (RDV53_06570) lgt 1329218..1330027 (+) 810 WP_005695515.1 prolipoprotein diacylglyceryl transferase -
  RDV53_RS06575 (RDV53_06575) - 1330038..1330889 (+) 852 WP_005695516.1 thymidylate synthase -
  RDV53_RS06580 (RDV53_06580) tadA 1330899..1331417 (+) 519 WP_005695517.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15151.42 Da        Isoelectric Point: 8.4674

>NTDB_id=874448 RDV53_RS06550 WP_005697917.1 1326188..1326631(-) (pilA) [Haemophilus parainfluenzae ATCC 33392 strain DSM 8978]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=874448 RDV53_RS06550 WP_005697917.1 1326188..1326631(-) (pilA) [Haemophilus parainfluenzae ATCC 33392 strain DSM 8978]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTACAAGCTTCGGCAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGCACCAATGCCCCGACAAACTGTTCTGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGTTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGTGC
ATTAGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTTACCTGGACAACCGCTTGCCCAAGCA
ATGCGGATTTATTCCCTGCGGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369YN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367