Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RBI10_RS16115 Genome accession   NZ_CP133047
Coordinates   3394941..3396653 (+) Length   570 a.a.
NCBI ID   WP_069120450.1    Uniprot ID   -
Organism   Acinetobacter pittii strain A1269     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3389941..3401653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBI10_RS16080 (RBI10_16115) nusA 3390829..3392313 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  RBI10_RS16085 (RBI10_16120) rimP 3392351..3392875 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  RBI10_RS16105 (RBI10_16140) secG 3393513..3393842 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  RBI10_RS16110 (RBI10_16145) tpiA 3393855..3394649 (-) 795 WP_032063691.1 triose-phosphate isomerase -
  RBI10_RS16115 (RBI10_16150) pilB 3394941..3396653 (+) 1713 WP_069120450.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RBI10_RS16120 (RBI10_16155) pilC 3396680..3397906 (+) 1227 WP_002114869.1 type II secretion system F family protein Machinery gene
  RBI10_RS16125 (RBI10_16160) pilD 3397906..3398766 (+) 861 WP_032070754.1 A24 family peptidase Machinery gene
  RBI10_RS16130 (RBI10_16165) coaE 3398768..3399364 (+) 597 WP_005065869.1 dephospho-CoA kinase -
  RBI10_RS16135 (RBI10_16170) - 3399361..3400275 (-) 915 WP_032063689.1 DMT family transporter -
  RBI10_RS16140 (RBI10_16175) rlmB 3400311..3401060 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RBI10_RS16145 (RBI10_16180) - 3401163..3401489 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63493.82 Da        Isoelectric Point: 4.9189

>NTDB_id=871466 RBI10_RS16115 WP_069120450.1 3394941..3396653(+) (pilB) [Acinetobacter pittii strain A1269]
MSVLNTSPKFSGFFRRLVEEKDVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=871466 RBI10_RS16115 WP_069120450.1 3394941..3396653(+) (pilB) [Acinetobacter pittii strain A1269]
ATGTCAGTCTTAAATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAAGATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCAGAAACAATTTCTATTGAATTTGCGGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCCCCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGCGTGTTGCGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGACGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCAACCCTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCTACCGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCAA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.3

100

0.519
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.016

100

0.377