NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RA306_RS07155	Genome accession	NZ_CP132898
Coordinates	1437308..1437742 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain P1933
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1432308..1442742

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RA306_RS07125 (RA306_07120)	-	1432872..1433141 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RA306_RS07130 (RA306_07125)	yacG	1433141..1433344 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RA306_RS07135 (RA306_07130)	coaE	1433355..1433975 (-)	621	WP_014326298.1	dephospho-CoA kinase	-
RA306_RS07140 (RA306_07135)	-	1433990..1434712 (-)	723	WP_014326299.1	A24 family peptidase	-
RA306_RS07145 (RA306_07140)	-	1434712..1435923 (-)	1212	WP_014326300.1	type II secretion system F family protein	-
RA306_RS07150 (RA306_07145)	pilB	1435923..1437308 (-)	1386	WP_016504529.1	GspE/PulE family protein	Machinery gene
RA306_RS07155 (RA306_07150)	pilA	1437308..1437742 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RA306_RS07160 (RA306_07155)	ampD	1437901..1438467 (+)	567	WP_014326302.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RA306_RS07165 (RA306_07160)	rppH	1439053..1439649 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RA306_RS07170 (RA306_07165)	-	1439652..1440447 (+)	796	Protein_1388	sulfite exporter TauE/SafE family protein	-
RA306_RS07175 (RA306_07170)	lgt	1440457..1441260 (+)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
RA306_RS07180 (RA306_07175)	-	1441266..1442117 (+)	852	WP_005751072.1	thymidylate synthase	-
RA306_RS07185 (RA306_07180)	tadA	1442132..1442632 (+)	501	WP_016504277.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=870493 RA306_RS07155 WP_005741096.1 1437308..1437742(-) (pilA) [Pasteurella multocida strain P1933]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=870493 RA306_RS07155 WP_005741096.1 1437308..1437742(-) (pilA) [Pasteurella multocida strain P1933]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC

CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0870493