Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5625_RS19455 Genome accession   NZ_AP024609
Coordinates   4321623..4323329 (+) Length   568 a.a.
NCBI ID   WP_221051585.1    Uniprot ID   -
Organism   Shewanella algae strain ATCC 49138     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4316623..4328329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5625_RS19430 (TUM3811_38540) pdhR 4316815..4317567 (-) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K5625_RS19435 (TUM3811_38550) ampE 4317842..4318693 (-) 852 WP_146509497.1 beta-lactamase regulator AmpE -
  K5625_RS19440 (TUM3811_38560) ampD 4318747..4319292 (-) 546 WP_025012051.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5625_RS19445 (TUM3811_38570) nadC 4319484..4320344 (+) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5625_RS19450 (TUM3811_38580) - 4321201..4321623 (+) 423 WP_144133982.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5625_RS19455 (TUM3811_38590) pilB 4321623..4323329 (+) 1707 WP_221051585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5625_RS19460 (TUM3811_38600) pilC 4323488..4324753 (+) 1266 WP_144133986.1 type II secretion system F family protein Machinery gene
  K5625_RS19465 (TUM3811_38610) pilD 4324829..4325743 (+) 915 WP_221051586.1 A24 family peptidase Machinery gene
  K5625_RS19470 (TUM3811_38620) coaE 4325744..4326355 (+) 612 WP_025012056.1 dephospho-CoA kinase -
  K5625_RS19475 (TUM3811_38630) zapD 4326352..4327092 (+) 741 WP_028779562.1 cell division protein ZapD -
  K5625_RS19480 (TUM3811_38640) yacG 4327137..4327349 (+) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5625_RS19485 (TUM3811_38650) mutT 4327349..4327747 (+) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63157.30 Da        Isoelectric Point: 5.3519

>NTDB_id=86494 K5625_RS19455 WP_221051585.1 4321623..4323329(+) (pilB) [Shewanella algae strain ATCC 49138]
MPTSGLHLGLSTLFIRKGLLTESQLTDTVKQSQKNRHSLVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLELDGLDEES
LAGIEVTDTDKRQQEEGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINTKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEEIPEHELLQLGFT
QQQIDAGFTPYKPCGCELCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86494 K5625_RS19455 WP_221051585.1 4321623..4323329(+) (pilB) [Shewanella algae strain ATCC 49138]
ATGCCTACATCGGGTCTTCATCTGGGATTATCGACTCTTTTTATCCGCAAAGGTTTGCTGACTGAAAGTCAGTTAACTGA
TACAGTCAAACAATCCCAGAAAAACAGACATTCACTGGTCACCACTCTGGTGACCAGTCAACTTTTGTCAGCACGAACTA
TTGCCGAACTCTGCTATGAAGAATACGGCACTCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATTCCGGAAGAG
TTTCTCAATAAAAAACTGATCGAAAAACACCGCTGCCTGCCGTTATTCAAGCGTGGCAACCGCCTCTATATCGCCACTTC
TGACCCCACCAATATCGCCGCCCTGGAGGACTTTCAGTTCAGCGCAGGCCTGCACGCCGAAGCGATACTGGTGGAGGAAG
ATAAACTCAGTAAAGCACTGGAAAAAGTGCTCGAAGAAGACTTGAGTTCACTGGAACTGGATGGTCTCGATGAGGAGTCT
CTCGCAGGTATCGAGGTCACCGACACCGACAAGCGTCAACAGGAAGAGGGTGAAAGTAAAGATGACGCCCCCATAGTCAT
CTATATCAACAAGATTTTGACCGATGCCATCCGTAAGGGGGCATCGGATCTGCATTTCGAGCCTTACGAAAAACGCTACC
GCATTCGCTTTCGTATCGATGGCATCCTCCACGAAGTATCTGAGCCACCCATTTCCCTCTCAGGCCGTATCTCAGCCCGC
TTGAAGGTAATGTCCAAACTTGATATCGCCGAACGCCGGGTCCCTCAGGATGGCCGCATCAAGATGAGGCTGTCCCGCAA
CAAGAGTATCGATTTTCGGGTCAGTACTCTGCCCACCCTTTGGGGTGAAAAAATAGTGATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGACCCCAG
GGGATGATTCTGGTAACCGGCCCTACCGGCTCAGGCAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCTGAAGACCCAGTGGAGATCAACCTGGAAGGGGTCAACCAAGTGCATATCAACACCAAGG
CCGGACTGACCTTCGCCTCGGCGCTGCGCTCCTTTTTACGTCAGGATCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATAGCCATTAAGGCGGCACAAACCGGCCACTTGGTACTCTCGACCCTGCATACCAACTCATCGGC
CGAAACCCTCACCCGCCTGCTCAACATGGGTGTCCCTGGTTATAACATTGCCAGTTCAGTCAATCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGTCAACCGGAAGAGATCCCTGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCTTACAAGCCCTGTGGCTGCGAGCTCTGCTCCGGTGGCTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATAGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCA
CCACCGCCAAGCAGCAAGGGATGCGCGATCTGCGCCTTTCTGGCCTTTTAAAAGTAATTCAAGGCGTCACCAGCATTGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.467

98.768

0.577
  pilB Acinetobacter baumannii D1279779

56.966

99.824

0.569
  pilB Legionella pneumophila strain ERS1305867

55.654

99.648

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

53.697

100

0.537
  pilB Vibrio campbellii strain DS40M4

52.482

99.296

0.521
  pilB Vibrio cholerae strain A1552

53.261

97.183

0.518
  pilF Neisseria gonorrhoeae MS11

48.319

99.472

0.481
  pilF Thermus thermophilus HB27

37.589

99.296

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.681

97.183

0.366

Multiple sequence alignment