Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHO37_RS01935 Genome accession   NZ_CP148116
Coordinates   418174..418851 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli isolate FELIX_MS508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413174..423851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO37_RS01920 chiA 414541..417234 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  WHO37_RS01925 bfd 417403..417597 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHO37_RS01930 bfr 417669..418145 (+) 477 WP_000675504.1 bacterioferritin -
  WHO37_RS01935 pilD 418174..418851 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  WHO37_RS01940 gspM 418851..419312 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  WHO37_RS01945 gspL 419309..420472 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  WHO37_RS01950 gspK 420487..421470 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WHO37_RS01955 gspJ 421463..422050 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WHO37_RS01960 gspI 422043..422420 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WHO37_RS01965 gspH 422417..422926 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  WHO37_RS01970 gspG 422934..423371 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=864865 WHO37_RS01935 WP_000178154.1 418174..418851(-) (pilD) [Escherichia coli isolate FELIX_MS508]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=864865 WHO37_RS01935 WP_000178154.1 418174..418851(-) (pilD) [Escherichia coli isolate FELIX_MS508]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378