Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q2K57_RS14465 Genome accession   NZ_CP130701
Coordinates   3215908..3217677 (-) Length   589 a.a.
NCBI ID   WP_304525504.1    Uniprot ID   -
Organism   Halomonas sp. I5-271120     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3210908..3222677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2K57_RS14440 - 3211816..3212727 (-) 912 WP_304525500.1 DNA replication terminus site-binding protein -
  Q2K57_RS14445 yacG 3212740..3212967 (-) 228 WP_112055800.1 DNA gyrase inhibitor YacG -
  Q2K57_RS14450 coaE 3213061..3213672 (-) 612 WP_304525501.1 dephospho-CoA kinase -
  Q2K57_RS14455 pilD 3213669..3214520 (-) 852 WP_304525502.1 A24 family peptidase Machinery gene
  Q2K57_RS14460 pilC 3214614..3215849 (-) 1236 WP_369700270.1 type II secretion system F family protein Machinery gene
  Q2K57_RS14465 pilB 3215908..3217677 (-) 1770 WP_304525504.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q2K57_RS18375 - 3218064..3218444 (+) 381 WP_369700271.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q2K57_RS14475 - 3218831..3220096 (+) 1266 WP_304525505.1 O-antigen ligase -
  Q2K57_RS14480 - 3220470..3221657 (-) 1188 WP_304525506.1 GGDEF domain-containing protein -
  Q2K57_RS14485 - 3221621..3222211 (-) 591 WP_304525507.1 hypothetical protein -

Sequence

Protein


Download         Length: 589 a.a.        Molecular weight: 64700.09 Da        Isoelectric Point: 5.3328

>NTDB_id=861101 Q2K57_RS14465 WP_304525504.1 3215908..3217677(-) (pilB) [Halomonas sp. I5-271120]
MQESQPRGAANHGLPAKDAGVASSLRGLALRLVADGQLTEAAALRAVQEAEEEDVSLMRHVVENGLVSARQTALSAAWEY
GLPLIDLDALRLASLPPPQEMPEKILRRLTVMPLYRTEHRLTVAVPYPSTLALLDELQFATNLKVEGILAPVDQLGPVLE
SYLSHHENAELFEGLDGADDALEGLEYEEYDPEDDQAITAGSDDAPVVKFVNKVLLDAIRRGASDIHFEPYESSYRIRMR
IDGILIEAARPPFGMRTRIAARLKVMSRLDISERRLPQDGAVKLKLSKQRSIDFRVSSLPTVFGEKLVLRILDPTTAQMG
IDSLGFTPPQRKMYEQALSEPQGMILVTGPTGSGKTVSLYTGINIINTGERNISTAEDPVEIKVPGVNQVNVLPKIGLTF
ASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLSNMGIASFNIASSVSLIIAQRLARR
LCKHCKEPVSLPAEVLREEGFNEDEIQQATIYHPVGCRHCTLGYKGRVGIYEVVPISDTISQLIMRNGNALELDEQARRE
GHPNLRRSGLLKVMQGMTSLEEVNRIIRD

Nucleotide


Download         Length: 1770 bp        

>NTDB_id=861101 Q2K57_RS14465 WP_304525504.1 3215908..3217677(-) (pilB) [Halomonas sp. I5-271120]
ATGCAGGAATCACAGCCGCGGGGAGCAGCCAATCATGGCCTTCCAGCCAAGGATGCCGGCGTAGCCTCATCGCTGCGGGG
GTTAGCGCTGCGCCTGGTGGCTGACGGCCAACTGACTGAAGCTGCCGCTCTGCGGGCCGTGCAGGAAGCCGAAGAAGAAG
ACGTCTCGCTGATGCGCCACGTGGTCGAGAACGGCCTGGTGTCGGCGCGTCAGACGGCGCTGTCGGCGGCCTGGGAGTAT
GGACTGCCGCTGATCGACCTGGACGCTCTTAGACTAGCCAGCCTGCCGCCACCCCAGGAAATGCCAGAAAAGATCCTGCG
CCGGCTCACCGTGATGCCGCTGTATCGCACCGAGCACCGCCTGACCGTGGCGGTGCCCTACCCGTCGACGCTTGCCTTGC
TGGACGAGCTGCAATTCGCCACCAATCTGAAGGTCGAGGGCATTCTGGCCCCGGTGGATCAGCTGGGGCCAGTGCTCGAG
AGCTACCTCAGCCATCACGAAAATGCCGAGTTGTTCGAAGGTCTGGACGGCGCCGACGATGCTCTGGAGGGGCTCGAGTA
CGAGGAATACGATCCTGAAGACGACCAGGCCATTACCGCTGGCAGCGACGATGCACCGGTAGTCAAGTTCGTCAACAAGG
TACTGTTGGACGCGATACGGCGCGGGGCCTCGGACATCCACTTCGAACCCTACGAATCAAGCTACCGAATTCGCATGCGC
ATCGATGGCATCCTGATCGAGGCTGCACGCCCTCCCTTCGGCATGCGCACCCGCATCGCTGCTCGCTTGAAAGTCATGTC
ACGGTTGGATATCTCCGAGCGCCGCCTGCCTCAGGACGGCGCCGTCAAGCTGAAACTCTCCAAGCAACGCTCGATCGACT
TCCGCGTCAGTTCTCTGCCGACAGTGTTCGGCGAAAAGTTGGTGCTGCGCATCCTCGACCCTACCACCGCCCAGATGGGC
ATCGACTCACTGGGTTTCACACCACCGCAGCGAAAGATGTACGAGCAGGCGCTCTCAGAGCCTCAAGGCATGATCCTGGT
CACCGGCCCCACCGGCAGCGGTAAAACGGTATCGCTGTATACCGGCATCAACATCATCAACACCGGTGAGCGCAACATTT
CTACCGCCGAGGACCCCGTAGAGATCAAGGTACCTGGCGTCAACCAGGTCAACGTGCTTCCAAAGATTGGCCTGACCTTC
GCCAGCGCTCTTCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTCATGGTCGGCGAGATTCGCGACCTGGAAACGGCGGA
GATCGCAGTCAAGGCCTCGCAGACCGGCCACCTGGTGCTATCCACCGTGCACACCAACTCGGCCGCAGAGACCCTGACAC
GCCTGTCGAATATGGGTATCGCATCGTTCAACATTGCAAGTTCGGTGTCGCTGATCATCGCCCAGCGTCTGGCGCGCCGC
CTGTGCAAGCACTGCAAGGAACCGGTGAGCCTCCCGGCAGAAGTGCTTCGAGAAGAAGGCTTCAATGAGGATGAAATCCA
GCAGGCGACCATCTACCACCCGGTCGGCTGCCGACATTGCACGCTTGGCTACAAGGGACGCGTCGGCATCTATGAAGTCG
TGCCTATCAGCGACACCATCAGTCAGCTGATTATGCGCAATGGCAACGCCCTCGAGCTTGACGAGCAAGCACGCCGCGAA
GGCCATCCGAATCTGCGCCGCAGTGGCCTTCTCAAGGTCATGCAAGGAATGACCAGCCTTGAGGAGGTCAACCGCATTAT
TCGGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(213-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(62-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.968

96.265

0.52
  pilB Acinetobacter baylyi ADP1

53.901

95.756

0.516
  pilB Acinetobacter baumannii D1279779

51.601

95.416

0.492
  pilB Vibrio campbellii strain DS40M4

47.652

97.623

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

48.649

94.228

0.458
  pilB Vibrio cholerae strain A1552

51.961

86.587

0.45
  pilF Neisseria gonorrhoeae MS11

50.719

82.683

0.419