Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q3Y66_RS08945 Genome accession   NZ_CP130610
Coordinates   1906386..1908158 (-) Length   590 a.a.
NCBI ID   WP_008956858.1    Uniprot ID   G4F3I2
Organism   Halomonas sp. HAL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1901386..1913158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3Y66_RS08920 (Q3Y66_08920) - 1902325..1903212 (-) 888 WP_035586313.1 DNA replication terminus site-binding protein -
  Q3Y66_RS08925 (Q3Y66_08925) yacG 1903252..1903485 (-) 234 WP_008956862.1 DNA gyrase inhibitor YacG -
  Q3Y66_RS08930 (Q3Y66_08930) coaE 1903519..1904121 (-) 603 WP_008956861.1 dephospho-CoA kinase -
  Q3Y66_RS08935 (Q3Y66_08935) pilD 1904231..1905076 (-) 846 WP_008956860.1 A24 family peptidase Machinery gene
  Q3Y66_RS08940 (Q3Y66_08940) pilC 1905153..1906382 (-) 1230 WP_008956859.1 type II secretion system F family protein Machinery gene
  Q3Y66_RS08945 (Q3Y66_08945) pilB 1906386..1908158 (-) 1773 WP_008956858.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q3Y66_RS08950 (Q3Y66_08950) - 1908540..1908947 (+) 408 WP_008956857.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q3Y66_RS08955 (Q3Y66_08955) - 1909183..1909632 (+) 450 WP_008956855.1 anti-virulence regulator CigR family protein -
  Q3Y66_RS08960 (Q3Y66_08960) - 1909727..1910542 (+) 816 WP_008956854.1 inositol monophosphatase family protein -
  Q3Y66_RS08970 (Q3Y66_08970) - 1910905..1911507 (+) 603 WP_035586310.1 DUF1819 family protein -
  Q3Y66_RS08975 (Q3Y66_08975) - 1911636..1912259 (+) 624 WP_008956852.1 DUF1788 domain-containing protein -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64553.99 Da        Isoelectric Point: 6.2070

>NTDB_id=860639 Q3Y66_RS08945 WP_008956858.1 1906386..1908158(-) (pilB) [Halomonas sp. HAL1]
MSQATTESTLSYESTLSHAAARGGQRGIAQRLIKHGLLTETQAAAAASAAVENDISLLEHVVESGLVPPTAAAVAAGYEY
GLPVINLEAVRISSLPPVTDYPVRILQSLNVVPLARHGHRLTVAVPYPATLTQLDELQFATGLSIEGILAPMDQLATTLS
SYLALTQSERGMLDEFNDLDDELSTLSVAEDSEVPLEEAPQNSDDGPIVKFVNKILLDAIRRGASDIHFEPYETQYRIRF
RIDGILIEVARPPLGMRNRVAARLKIMARLDISERRLPQDGAIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPSATQL
GIEQLGFTDDQRHFYERALNQPQGMILVSGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLD
FASALRAFLRQDPDVVMVGEIRDLETADIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVTSFNIASAVSLIIAQRLTR
KLCPRCKEPIVIPHEALRKEGFSDEEIQQATIFKPVGCKHCTHGYKGRVGIYEVVPITEAMRQLIMSNANAMDIDQQARR
EGYPSLHQSGLLKVMRGITSLEEINRVSKE

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=860639 Q3Y66_RS08945 WP_008956858.1 1906386..1908158(-) (pilB) [Halomonas sp. HAL1]
ATGAGCCAAGCGACTACCGAATCAACCTTAAGTTATGAGTCAACCCTAAGCCACGCCGCTGCACGGGGCGGGCAGCGCGG
TATTGCCCAGCGTTTAATCAAGCACGGCTTGCTAACCGAAACGCAGGCTGCGGCGGCCGCTAGCGCCGCCGTGGAAAATG
ATATTTCACTATTAGAGCACGTGGTTGAAAGCGGGCTTGTGCCCCCCACTGCCGCGGCGGTTGCCGCGGGGTACGAGTAC
GGGCTGCCGGTTATCAATTTAGAAGCCGTTCGTATTAGCTCGCTGCCTCCTGTTACTGACTACCCAGTGAGGATTCTGCA
AAGCTTAAATGTGGTGCCTCTGGCACGTCATGGGCACCGTTTAACCGTGGCCGTGCCTTACCCCGCCACGCTTACCCAGC
TTGATGAGTTACAGTTTGCCACCGGGCTTAGCATTGAAGGGATATTAGCCCCGATGGACCAGCTAGCCACCACGCTGAGT
AGCTATTTAGCATTAACGCAAAGCGAACGTGGCATGCTGGACGAGTTTAATGACCTCGATGATGAGTTATCAACGCTGAG
TGTTGCCGAAGACAGTGAAGTGCCTTTGGAAGAAGCACCTCAAAACAGTGATGATGGCCCCATCGTCAAGTTTGTTAATA
AGATTTTATTAGATGCGATTCGCCGTGGCGCGTCCGACATTCATTTCGAGCCTTACGAAACGCAGTATCGTATACGTTTT
CGTATTGACGGCATCTTAATTGAGGTCGCACGCCCTCCCTTAGGGATGCGTAACCGTGTTGCCGCCCGCTTGAAAATTAT
GGCGCGGCTGGATATTTCTGAGCGGCGCTTACCCCAAGACGGGGCCATAAAACTTAAATTATCGCGTACGCGCTCGCTCG
ATTTTCGGGTCAACTCCTTGCCCACGGTGTATGGCGAAAAATTGGTATTGCGTATTCTCGACCCTTCCGCGACCCAACTC
GGCATCGAGCAATTAGGCTTTACTGATGACCAACGTCACTTTTATGAACGTGCCCTCAACCAGCCCCAGGGGATGATATT
GGTGTCGGGCCCCACTGGCAGTGGCAAAACCGTCTCGCTGTATACCGGCATTAATATTCTCAATAAAGTGGAGCGCAATA
TTTGCACGGCGGAAGATCCGGTTGAGATTAAAGTAGCCGGTATTAATCAGGTCAATGTGTTGCCTAAAATTGGGCTTGAT
TTTGCTAGTGCGCTACGGGCATTTCTGCGCCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGTGATTTAGAAACCGC
TGACATTGCCGTTAAAGCCTCCCAGACAGGCCACTTGGTGCTTTCTACCGTGCACACTAACTCAGCTGCCGAAACGCTCA
CGCGGCTGGCGAACATGGGGGTTACTTCGTTTAACATCGCCAGCGCCGTTAGCCTGATTATTGCCCAACGCTTAACGCGC
AAACTCTGCCCACGCTGCAAGGAGCCGATTGTTATCCCCCACGAGGCACTGCGCAAAGAGGGCTTTAGCGATGAAGAAAT
CCAACAGGCCACTATTTTCAAGCCGGTAGGTTGTAAGCACTGCACGCATGGCTATAAAGGTCGCGTGGGTATTTATGAGG
TCGTTCCCATTACGGAAGCCATGCGCCAATTAATTATGAGCAACGCCAACGCGATGGATATCGATCAGCAGGCGCGCCGC
GAAGGCTACCCCAGTTTGCACCAGAGCGGTTTATTAAAAGTCATGCGGGGGATTACCAGCCTTGAAGAAATTAACCGCGT
TAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-164)

Type II/IV secretion system protein (T2SSE)

(214-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4F3I2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.327

95.763

0.492
  pilB Acinetobacter baumannii D1279779

49.912

96.102

0.48
  pilB Acinetobacter baylyi ADP1

49.647

95.932

0.476
  pilB Vibrio campbellii strain DS40M4

51.005

92.712

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

49.027

95.763

0.469
  pilB Vibrio cholerae strain A1552

49.097

93.898

0.461
  pilF Neisseria gonorrhoeae MS11

49.792

81.356

0.405