Detailed information NTG0860637

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Q3Y66_RS08935
• Genome accession: NZ_CP130610
• Coordinates: 1904231..1905076 (-)
• Length: 281 a.a.
• NCBI ID: WP_008956860.1
• Uniprot ID: G4F3I4
• Organism: Halomonas sp. HAL1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1899231..1910076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q3Y66_RS08900 (Q3Y66_08900)	-	1899633..1900139 (-)	507	WP_238528482.1	hypothetical protein	-
Q3Y66_RS08905 (Q3Y66_08905)	-	1900179..1900490 (-)	312	Protein_1750	helix-turn-helix transcriptional regulator	-
Q3Y66_RS08910 (Q3Y66_08910)	-	1900852..1901196 (+)	345	WP_008957330.1	hypothetical protein	-
Q3Y66_RS08915 (Q3Y66_08915)	-	1901285..1902298 (+)	1014	WP_303319479.1	IS630 family transposase	-
Q3Y66_RS08920 (Q3Y66_08920)	-	1902325..1903212 (-)	888	WP_035586313.1	DNA replication terminus site-binding protein	-
Q3Y66_RS08925 (Q3Y66_08925)	yacG	1903252..1903485 (-)	234	WP_008956862.1	DNA gyrase inhibitor YacG	-
Q3Y66_RS08930 (Q3Y66_08930)	coaE	1903519..1904121 (-)	603	WP_008956861.1	dephospho-CoA kinase	-
Q3Y66_RS08935 (Q3Y66_08935)	pilD	1904231..1905076 (-)	846	WP_008956860.1	A24 family peptidase	Machinery gene
Q3Y66_RS08940 (Q3Y66_08940)	pilC	1905153..1906382 (-)	1230	WP_008956859.1	type II secretion system F family protein	Machinery gene
Q3Y66_RS08945 (Q3Y66_08945)	pilB	1906386..1908158 (-)	1773	WP_008956858.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Q3Y66_RS08950 (Q3Y66_08950)	-	1908540..1908947 (+)	408	WP_008956857.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
Q3Y66_RS08955 (Q3Y66_08955)	-	1909183..1909632 (+)	450	WP_008956855.1	anti-virulence regulator CigR family protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 30798.10 Da
• Isoelectric Point: 7.9079

>NTDB_id=860637 Q3Y66_RS08935 WP_008956860.1 1904231..1905076(-) (pilD) [Halomonas sp. HAL1]
MHYPPLIVWLMALLVGLCLGSFLNVVITRLPVMLMRHWRREARAALELDEEHSPRFNLATPGSLCPRCETPIAWHDNLPL
IGWIKRRGRCAGCQTSISVQYPLVEMAGGLLALAVVALHGLTAESLFIYGACLMLLALAVIDFRTQLLPDVITLPLLWAG
LLFQLLFQPFMLSDAVIGVMVGYLSLWSFYWLFKLVTGKEGMGFGDFKLLAALGAWLGWNFLPLILILSAGLGAVVGLTA
QACAPRLRGKPLPFGPFLALAGWVALLVGDELMALYLSLLS

Nucleotide

• Length: 846 bp

>NTDB_id=860637 Q3Y66_RS08935 WP_008956860.1 1904231..1905076(-) (pilD) [Halomonas sp. HAL1]
ATGCATTACCCTCCCCTGATTGTATGGCTCATGGCTTTATTGGTTGGGCTGTGCTTAGGCAGTTTTCTTAATGTTGTTAT
CACGCGCCTACCGGTCATGCTGATGCGCCATTGGCGGCGTGAGGCGCGTGCAGCGCTTGAACTGGATGAGGAACACTCGC
CACGCTTCAATTTAGCCACACCAGGCTCCCTGTGCCCCCGCTGTGAAACCCCGATTGCCTGGCACGATAACCTGCCGCTG
ATTGGCTGGATTAAACGCCGCGGGCGGTGTGCAGGGTGTCAAACGAGCATCAGCGTTCAATATCCGCTGGTGGAAATGGC
CGGTGGCCTACTGGCCTTGGCGGTAGTGGCACTCCATGGCCTCACTGCAGAGTCGTTGTTTATTTACGGCGCCTGCTTGA
TGCTGTTAGCACTCGCCGTGATCGATTTTCGTACCCAGTTATTGCCGGATGTTATTACGCTCCCGCTGCTATGGGCAGGC
TTACTTTTTCAGCTACTCTTCCAGCCGTTTATGCTCTCCGATGCCGTTATCGGCGTGATGGTGGGCTATCTTTCTCTATG
GAGTTTTTATTGGCTGTTCAAACTCGTCACCGGTAAAGAGGGCATGGGCTTTGGCGACTTTAAGCTGCTGGCAGCATTGG
GCGCCTGGCTAGGCTGGAACTTTTTACCACTTATCTTGATACTTTCCGCAGGGCTTGGCGCGGTGGTTGGTTTAACTGCG
CAGGCCTGCGCGCCTCGCTTACGTGGCAAACCATTACCCTTTGGGCCTTTTCTTGCCCTCGCTGGTTGGGTAGCGTTATT
AGTTGGCGATGAGCTAATGGCGCTGTATCTCAGCCTGCTTAGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-116)

• Type IV leader peptidase family (Peptidase_A24) (132-238)

3D structure

• AlphaFold DB: G4F3I4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.648	100	0.502
pilD	Acinetobacter baumannii D1279779	47.407	96.085	0.456
pilD	Acinetobacter nosocomialis M2	47.407	96.085	0.456
pilD	Vibrio campbellii strain DS40M4	44.84	100	0.448
pilD	Neisseria gonorrhoeae MS11	42.803	93.95	0.402