Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   LPP_RS00915 Genome accession   NC_006368
Coordinates   212387..213196 (+) Length   269 a.a.
NCBI ID   WP_011212818.1    Uniprot ID   -
Organism   Legionella pneumophila str. Paris     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 207387..218196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS00890 (lpp0182) - 207419..208168 (+) 750 WP_011212813.1 hypothetical protein -
  LPP_RS00895 (lpp0183) traA 208292..210931 (-) 2640 WP_011212814.1 Ti-type conjugative transfer relaxase TraA -
  LPP_RS00900 (lpp0184) - 211180..211386 (+) 207 WP_011212815.1 hypothetical protein -
  LPP_RS00905 (lpp0185) - 211373..211642 (+) 270 WP_011212816.1 conjugal transfer protein TraD -
  LPP_RS00910 (lpp0186) - 211736..212383 (+) 648 WP_011212817.1 hypothetical protein -
  LPP_RS00915 (lpp0187) comEC 212387..213196 (+) 810 WP_011212818.1 ComEC/Rec2 family competence protein Machinery gene
  LPP_RS00920 (lpp0188) - 213223..213684 (-) 462 WP_011212819.1 nucleoside deaminase -
  LPP_RS00925 (lpp0189) - 213677..215104 (-) 1428 WP_011212820.1 VUT family protein -
  LPP_RS00930 (lpp0190) - 215097..215456 (-) 360 WP_011212821.1 hypothetical protein -
  LPP_RS00935 (lpp0191) - 215473..216120 (-) 648 WP_011212822.1 LexA family transcriptional regulator -
  LPP_RS00940 (lpp0192) - 216289..216528 (+) 240 WP_011212823.1 hypothetical protein -
  LPP_RS16370 (lpp0193) - 216559..216765 (+) 207 WP_011212824.1 hypothetical protein -
  LPP_RS00945 (lpp0194) - 216714..217922 (+) 1209 WP_011212825.1 tyrosine-type recombinase/integrase -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 30583.73 Da        Isoelectric Point: 7.2561

>NTDB_id=1269 LPP_RS00915 WP_011212818.1 212387..213196(+) (comEC) [Legionella pneumophila str. Paris]
MKLQIFDVEHGACALLTCINGTRLMIDCGHNANSGWKPGSYLRSQGVTHLDMLAITNYDEDHVSGLPDLLEQVHVNWLWR
NPSVSATDIKNMKAKNGMGYGIESLVNILPTYKYTGWQPPEFSFVERRGFYAPYPTFVDTNNLSMAIHLNIAGINFLFSG
DLESGGWNTLLKDQSFRNVIANTDIFIAPHHGRESGIYPQIFEEYQCFPHYIIMSDKSHMYDTQKTLDYYSSKARGGFFR
GKHRRVLTTRKDGSITFNFNFLKGECLVA

Nucleotide


Download         Length: 810 bp        

>NTDB_id=1269 LPP_RS00915 WP_011212818.1 212387..213196(+) (comEC) [Legionella pneumophila str. Paris]
GTGAAATTACAAATTTTTGATGTAGAACATGGTGCATGCGCACTCTTAACATGCATTAATGGCACAAGACTAATGATTGA
CTGTGGACATAACGCTAATAGTGGCTGGAAACCAGGTTCTTACTTGCGCAGTCAAGGAGTAACCCATCTCGATATGTTAG
CCATAACTAATTACGATGAAGATCACGTGAGTGGATTACCTGATTTACTTGAGCAAGTACATGTTAATTGGTTATGGCGT
AATCCATCAGTTTCAGCAACAGATATAAAAAATATGAAAGCCAAAAATGGAATGGGATATGGGATTGAAAGTTTAGTAAA
TATTCTCCCGACATATAAATATACAGGATGGCAACCGCCTGAGTTTTCATTTGTTGAAAGACGGGGTTTTTATGCTCCTT
ACCCTACTTTTGTAGACACGAATAATCTAAGCATGGCCATTCATCTTAATATTGCAGGAATAAATTTTTTGTTTTCAGGC
GATTTAGAATCAGGAGGATGGAATACTCTGTTAAAAGATCAGAGCTTTCGGAATGTGATTGCGAACACAGATATTTTTAT
AGCACCCCATCATGGACGAGAAAGTGGGATTTATCCCCAAATTTTCGAAGAATATCAGTGTTTCCCTCACTATATTATAA
TGTCCGATAAAAGCCATATGTACGATACACAAAAAACTCTAGATTACTATTCTAGTAAAGCACGCGGTGGTTTTTTTAGG
GGGAAACATCGTCGAGTTTTAACGACAAGAAAGGATGGTTCAATAACCTTTAATTTTAATTTTTTAAAGGGGGAATGTCT
TGTAGCTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]