Detailed information NTG0860603

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: Q3Y59_RS12385
• Genome accession: NZ_CP130608
• Coordinates: 2550326..2550940 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain XDY66
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545326..2555940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q3Y59_RS12355 (Q3Y59_12355)	rpsT	2545626..2545892 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
Q3Y59_RS12360 (Q3Y59_12360)	holA	2545909..2546950 (-)	1042	Protein_2390	DNA polymerase III subunit delta	-
Q3Y59_RS12365 (Q3Y59_12365)	-	2546990..2547144 (-)	155	Protein_2391	hypothetical protein	-
Q3Y59_RS12370 (Q3Y59_12370)	-	2547185..2547319 (+)	135	WP_003152870.1	YqzM family protein	-
Q3Y59_RS12375 (Q3Y59_12375)	comEC	2547338..2549689 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
Q3Y59_RS12380 (Q3Y59_12380)	-	2549690..2550259 (-)	570	WP_003152868.1	ComE operon protein 2	-
Q3Y59_RS12385 (Q3Y59_12385)	comEA	2550326..2550940 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
Q3Y59_RS12390 (Q3Y59_12390)	comER	2550999..2551820 (+)	822	WP_014305449.1	late competence protein ComER	-
Q3Y59_RS12395 (Q3Y59_12395)	-	2551889..2552626 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
Q3Y59_RS12400 (Q3Y59_12400)	rsfS	2552623..2552979 (-)	357	WP_003152864.1	ribosome silencing factor	-
Q3Y59_RS12405 (Q3Y59_12405)	yqeK	2552976..2553557 (-)	582	WP_057080474.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
Q3Y59_RS12410 (Q3Y59_12410)	-	2553547..2554116 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
Q3Y59_RS12415 (Q3Y59_12415)	yhbY	2554127..2554417 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
Q3Y59_RS12420 (Q3Y59_12420)	aroE	2554411..2555253 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=860603 Q3Y59_RS12385 WP_003152867.1 2550326..2550940(-) (comEA) [Bacillus velezensis strain XDY66]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=860603 Q3Y59_RS12385 WP_003152867.1 2550326..2550940(-) (comEA) [Bacillus velezensis strain XDY66]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363