Detailed information NTG0858222

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: Q3O83_RS12385
• Genome accession: NZ_CP130445
• Coordinates: 2550903..2551517 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain C6.7
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545903..2556517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q3O83_RS12355 (Q3O83_12355)	rpsT	2546203..2546469 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
Q3O83_RS12360 (Q3O83_12360)	holA	2546486..2547527 (-)	1042	Protein_2389	DNA polymerase III subunit delta	-
Q3O83_RS12365 (Q3O83_12365)	-	2547567..2547721 (-)	155	Protein_2390	hypothetical protein	-
Q3O83_RS12370 (Q3O83_12370)	-	2547762..2547896 (+)	135	WP_003152870.1	YqzM family protein	-
Q3O83_RS12375 (Q3O83_12375)	comEC	2547915..2550266 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
Q3O83_RS12380 (Q3O83_12380)	-	2550267..2550836 (-)	570	WP_003152868.1	ComE operon protein 2	-
Q3O83_RS12385 (Q3O83_12385)	comEA	2550903..2551517 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
Q3O83_RS12390 (Q3O83_12390)	comER	2551576..2552397 (+)	822	WP_014305449.1	late competence protein ComER	-
Q3O83_RS12395 (Q3O83_12395)	-	2552466..2553203 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
Q3O83_RS12400 (Q3O83_12400)	rsfS	2553200..2553556 (-)	357	WP_003152864.1	ribosome silencing factor	-
Q3O83_RS12405 (Q3O83_12405)	yqeK	2553553..2554134 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
Q3O83_RS12410 (Q3O83_12410)	-	2554124..2554693 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
Q3O83_RS12415 (Q3O83_12415)	yhbY	2554704..2554994 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
Q3O83_RS12420 (Q3O83_12420)	aroE	2554988..2555830 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=858222 Q3O83_RS12385 WP_003152867.1 2550903..2551517(-) (comEA) [Bacillus amyloliquefaciens strain C6.7]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=858222 Q3O83_RS12385 WP_003152867.1 2550903..2551517(-) (comEA) [Bacillus amyloliquefaciens strain C6.7]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363