Detailed information NTG0857564

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: Q2B68_RS13800
• Genome accession: NZ_CP130280
• Coordinates: 2791423..2792037 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain PM415
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2786423..2797037

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q2B68_RS13770 (Q2B68_13770)	rpsT	2786723..2786989 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
Q2B68_RS13775 (Q2B68_13775)	holA	2787006..2788047 (-)	1042	Protein_2674	DNA polymerase III subunit delta	-
Q2B68_RS13780 (Q2B68_13780)	-	2788087..2788241 (-)	155	Protein_2675	hypothetical protein	-
Q2B68_RS13785 (Q2B68_13785)	-	2788282..2788416 (+)	135	WP_003152870.1	YqzM family protein	-
Q2B68_RS13790 (Q2B68_13790)	comEC	2788435..2790786 (-)	2352	WP_302564960.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
Q2B68_RS13795 (Q2B68_13795)	-	2790787..2791356 (-)	570	WP_003152868.1	ComE operon protein 2	-
Q2B68_RS13800 (Q2B68_13800)	comEA	2791423..2792037 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
Q2B68_RS13805 (Q2B68_13805)	comER	2792096..2792917 (+)	822	WP_014305449.1	late competence protein ComER	-
Q2B68_RS13810 (Q2B68_13810)	-	2792986..2793723 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
Q2B68_RS13815 (Q2B68_13815)	rsfS	2793720..2794076 (-)	357	WP_003152864.1	ribosome silencing factor	-
Q2B68_RS13820 (Q2B68_13820)	yqeK	2794094..2794654 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
Q2B68_RS13825 (Q2B68_13825)	-	2794644..2795213 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
Q2B68_RS13830 (Q2B68_13830)	yhbY	2795224..2795514 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
Q2B68_RS13835 (Q2B68_13835)	aroE	2795508..2796350 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=857564 Q2B68_RS13800 WP_041482377.1 2791423..2792037(-) (comEA) [Bacillus amyloliquefaciens strain PM415]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=857564 Q2B68_RS13800 WP_041482377.1 2791423..2792037(-) (comEA) [Bacillus amyloliquefaciens strain PM415]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363