Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q2F48_RS02675 Genome accession   NZ_CP130164
Coordinates   543355..544464 (-) Length   369 a.a.
NCBI ID   WP_302097967.1    Uniprot ID   -
Organism   Aeromonas caviae strain WZDHY22015     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538355..549464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2F48_RS02650 (Q2F48_02650) - 538487..538714 (+) 228 WP_039039956.1 hypothetical protein -
  Q2F48_RS02655 (Q2F48_02655) brnQ 538805..540067 (-) 1263 WP_045523295.1 branched-chain amino acid transport system II carrier protein -
  Q2F48_RS02660 (Q2F48_02660) - 540303..541013 (-) 711 WP_201871603.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  Q2F48_RS02665 (Q2F48_02665) srmB 541141..542361 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  Q2F48_RS02670 (Q2F48_02670) yaaA 542549..543322 (-) 774 WP_069652225.1 peroxide stress protein YaaA -
  Q2F48_RS02675 (Q2F48_02675) pilU 543355..544464 (-) 1110 WP_302097967.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q2F48_RS02680 (Q2F48_02680) pilT 544488..545522 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  Q2F48_RS02685 (Q2F48_02685) - 545562..546263 (+) 702 WP_263308808.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q2F48_RS02690 (Q2F48_02690) proC 546386..547210 (+) 825 WP_039039961.1 pyrroline-5-carboxylate reductase -
  Q2F48_RS02695 (Q2F48_02695) - 547234..547785 (+) 552 WP_042014922.1 YggT family protein -
  Q2F48_RS02700 (Q2F48_02700) yggU 547785..548084 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  Q2F48_RS02705 (Q2F48_02705) - 548103..548522 (+) 420 WP_049636470.1 DUF4426 domain-containing protein -
  Q2F48_RS02710 (Q2F48_02710) - 548598..549428 (-) 831 WP_042864458.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40812.98 Da        Isoelectric Point: 6.4405

>NTDB_id=857422 Q2F48_RS02675 WP_302097967.1 543355..544464(-) (pilU) [Aeromonas caviae strain WZDHY22015]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHARCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=857422 Q2F48_RS02675 WP_302097967.1 543355..544464(-) (pilU) [Aeromonas caviae strain WZDHY22015]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGCTCGCTGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

58.074

95.664

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

91.057

0.393
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379