Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QUE78_RS09750 Genome accession   NZ_AP027424
Coordinates   1988176..1989186 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain B27 isolate B27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1983176..1994186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE78_RS09720 (MUTS8_18690) nudB 1984900..1985352 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  QUE78_RS09725 (MUTS8_18700) yebC 1985381..1986121 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QUE78_RS09730 (MUTS8_18710) ruvC 1986156..1986677 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QUE78_RS09735 (MUTS8_18720) yebB 1986679..1987281 (-) 603 WP_001024904.1 YebB family permuted papain-like enzyme -
  QUE78_RS09740 yobI 1987352..1987417 (+) 66 WP_072094247.1 stress response small protein YobI -
  QUE78_RS09745 (MUTS8_18730) ruvA 1987556..1988167 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QUE78_RS09750 (MUTS8_18740) ruvB 1988176..1989186 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QUE78_RS09755 (MUTS8_18750) znuB 1989333..1990118 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  QUE78_RS09760 (MUTS8_18760) znuC 1990115..1990870 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  QUE78_RS09765 (MUTS8_18770) znuA 1990949..1991881 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  QUE78_RS09770 (MUTS8_18780) mepM 1991897..1993219 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=85739 QUE78_RS09750 WP_000568519.1 1988176..1989186(+) (ruvB) [Escherichia coli strain B27 isolate B27]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=85739 QUE78_RS09750 WP_000568519.1 1988176..1989186(+) (ruvB) [Escherichia coli strain B27 isolate B27]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTTTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment