Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QYQ99_RS08060 Genome accession   NZ_CP129672
Coordinates   1754079..1755821 (+) Length   580 a.a.
NCBI ID   WP_302092187.1    Uniprot ID   -
Organism   Comamonas testosteroni strain MWF001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1749079..1760821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ99_RS08030 (QYQ99_08030) proB 1749422..1750561 (-) 1140 WP_302092184.1 glutamate 5-kinase -
  QYQ99_RS08035 (QYQ99_08035) cgtA 1750672..1751787 (-) 1116 WP_302092185.1 Obg family GTPase CgtA -
  QYQ99_RS08040 (QYQ99_08040) rpmA 1751944..1752201 (-) 258 WP_003058534.1 50S ribosomal protein L27 -
  QYQ99_RS08045 (QYQ99_08045) rplU 1752218..1752529 (-) 312 WP_003058536.1 50S ribosomal protein L21 -
  QYQ99_RS08050 (QYQ99_08050) - 1752895..1753824 (+) 930 WP_302092186.1 polyprenyl synthetase family protein -
  QYQ99_RS08060 (QYQ99_08060) pilB 1754079..1755821 (+) 1743 WP_302092187.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QYQ99_RS08065 (QYQ99_08065) pilC 1755846..1757063 (+) 1218 WP_302092188.1 type II secretion system F family protein Machinery gene
  QYQ99_RS08070 (QYQ99_08070) - 1757063..1757932 (+) 870 WP_302092189.1 A24 family peptidase -
  QYQ99_RS08075 (QYQ99_08075) coaE 1757936..1758562 (+) 627 WP_302092190.1 dephospho-CoA kinase -
  QYQ99_RS08080 (QYQ99_08080) zapD 1758650..1759405 (+) 756 WP_302092191.1 cell division protein ZapD -
  QYQ99_RS08085 (QYQ99_08085) - 1759505..1759714 (+) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  QYQ99_RS08090 (QYQ99_08090) - 1759727..1760197 (-) 471 WP_302092192.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63173.41 Da        Isoelectric Point: 5.1608

>NTDB_id=854847 QYQ99_RS08060 WP_302092187.1 1754079..1755821(+) (pilB) [Comamonas testosteroni strain MWF001]
MAAADNLQKNAPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDVQQLPRELLDPKISNAYRVLAISKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNTVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAISRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHEALLEAGYDEAELNGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=854847 QYQ99_RS08060 WP_302092187.1 1754079..1755821(+) (pilB) [Comamonas testosteroni strain MWF001]
ATGGCCGCTGCCGACAATCTGCAAAAGAACGCCCCTGCGCCCATCGCCATCCCTGGTCTAGGCAGAGCCCTGATTTCTGC
AGGAAAGGTTTCGCAGCAAGCCGCCGAGAACGCTGCCAAGAAAGCCGCCACAGGCAAGACCCCGTTCATTACCGAGCTCA
GCCAGTCTGGCGAAATCAGCGCGCTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACTCCGCTGCTGGACCTCAAC
GCCATCGACGTCCAGCAACTTCCGCGCGAGCTGCTGGACCCGAAAATCAGCAACGCCTATCGTGTGCTGGCAATCAGCAA
GCGCGGCAATCGGCTCACCATCGCCACGGCAGACCCCACTCAGCAGGAAGCTGCCGAACAGATCAAATTCACGACTCAGT
TACTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTCGTCGAGCAGACCGGCAAGAGCGTCAGCGAATCG
CTGGACTCCTATTCCAGCTCCGGAGACTTTGACTTTGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATAC
CGTCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCAT
CCGACTTGCATTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGCATCGACGGCGAACTGCGCGAGATAGCCTCT
CCTCCTATCGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAGGACGGCCGCATGAAGCTCAAGGTCGGCCCCGACCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCTCGGCCAAGATGGGCATCGACGCCCTGGGCTACGAGCCCGAGGAA
AAAGAGCGCCTGCTCAAGGCCATCAGCCGCCCCTACGGGATGATTCTGGTCACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTCTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACCGCCGAAGACCCCTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTCAATGTGAACGAGAAGGCTGGCCTGACCTTTGCTGCTGCGCTCAAATCCTTTCTGCGCCAG
GACCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGTCA
CCTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATTTTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGCGCGCAGTGCAAGGAACCTGCCGAC
ATACCGCATGAGGCCCTGCTCGAAGCGGGCTACGATGAAGCAGAACTCAACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCGGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATCTATCAGGTGATGCCCATCAGCGAAGAAATTCAACGCA
TCATCTTGCGTGACGGCAGCGCTCTGGAGATTGCCGAACAGGCAAAGGCCGAAGGAGTACGCTCTCTGCGTGGCTCGGGC
CTGCATAAAGTCAAGTTGGGTCTGACCTCACTCGAAGAAGTGCTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilB Acinetobacter baylyi ADP1

54.261

99.138

0.538
  pilF Neisseria gonorrhoeae MS11

52.65

97.586

0.514
  pilB Legionella pneumophila strain ERS1305867

50.883

97.586

0.497
  pilB Vibrio cholerae strain A1552

47.436

94.138

0.447
  pilB Vibrio campbellii strain DS40M4

43.85

96.724

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

46.008

90.69

0.417
  pilF Thermus thermophilus HB27

38.715

99.31

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.596

98.276

0.379