Detailed information NTG0085445

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVAD3_RS12535
• Genome accession: NZ_AP024501
• Coordinates: 2605764..2606378 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain AD-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2600764..2611378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVAD3_RS12505 (BVAD3_24880)	rpsT	2601067..2601333 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVAD3_RS12510 (BVAD3_24890)	holA	2601350..2602391 (-)	1042	Protein_2441	DNA polymerase III subunit delta	-
BVAD3_RS12515	-	2602431..2602582 (-)	152	Protein_2442	hypothetical protein	-
BVAD3_RS12520 (BVAD3_24900)	-	2602623..2602757 (+)	135	WP_003152870.1	YqzM family protein	-
BVAD3_RS12525 (BVAD3_24910)	comEC	2602776..2605127 (-)	2352	WP_163094353.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVAD3_RS12530 (BVAD3_24920)	-	2605128..2605697 (-)	570	WP_003152868.1	ComE operon protein 2	-
BVAD3_RS12535 (BVAD3_24930)	comEA	2605764..2606378 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVAD3_RS12540 (BVAD3_24940)	comER	2606437..2607258 (+)	822	WP_014418420.1	late competence protein ComER	-
BVAD3_RS12545 (BVAD3_24950)	-	2607327..2608064 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
BVAD3_RS12550 (BVAD3_24960)	rsfS	2608061..2608417 (-)	357	WP_007408260.1	ribosome silencing factor	-
BVAD3_RS12555 (BVAD3_24970)	yqeK	2608435..2608995 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVAD3_RS12560 (BVAD3_24980)	-	2608985..2609554 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BVAD3_RS12565 (BVAD3_24990)	yhbY	2609565..2609855 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BVAD3_RS12570 (BVAD3_25000)	aroE	2609849..2610691 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=85445 BVAD3_RS12535 WP_014418419.1 2605764..2606378(-) (comEA) [Bacillus velezensis strain AD-3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=85445 BVAD3_RS12535 WP_014418419.1 2605764..2606378(-) (comEA) [Bacillus velezensis strain AD-3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363