Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J3989_RS03245 Genome accession   NZ_AP024489
Coordinates   652762..654438 (+) Length   558 a.a.
NCBI ID   WP_002221967.1    Uniprot ID   A0A0Y5AAS9
Organism   Neisseria meningitidis strain NIID777     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647762..659438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3989_RS03200 (NMNIID777_05900) rpmB 647805..648038 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  J3989_RS03205 (NMNIID777_05910) rpmG 648067..648222 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  J3989_RS03210 (NMNIID777_05920) ubiM 648493..649677 (-) 1185 WP_101116484.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  J3989_RS03215 (NMNIID777_05930) rpmA 650066..650338 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  J3989_RS03220 (NMNIID777_05940) rplU 650363..650671 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  J3989_RS03230 (NMNIID777_05950) ispB 650895..651869 (+) 975 WP_061694686.1 octaprenyl diphosphate synthase -
  J3989_RS03235 (NMNIID777_05960) - 651874..652320 (+) 447 WP_002220271.1 DUF441 domain-containing protein -
  J3989_RS03245 (NMNIID777_05970) pilF 652762..654438 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J3989_RS03250 - 654523..654732 (-) 210 WP_002224872.1 DNA gyrase inhibitor YacG -
  J3989_RS03255 (NMNIID777_05980) coaE 654725..655357 (-) 633 WP_002254836.1 dephospho-CoA kinase -
  J3989_RS03260 (NMNIID777_05990) pilD 655359..656219 (-) 861 WP_002220276.1 A24 family peptidase Machinery gene
  J3989_RS03265 (NMNIID777_06000) pilG 656293..657525 (-) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61965.05 Da        Isoelectric Point: 5.3037

>NTDB_id=85340 J3989_RS03245 WP_002221967.1 652762..654438(+) (pilF) [Neisseria meningitidis strain NIID777]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=85340 J3989_RS03245 WP_002221967.1 652762..654438(+) (pilF) [Neisseria meningitidis strain NIID777]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAATTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGTGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGCCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCACGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Y5AAS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.462

100

0.995
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.728

0.375
  pilF Thermus thermophilus HB27

36.949

97.491

0.36

Multiple sequence alignment