Detailed information NTG0852094

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QYH60_RS06020
• Genome accession: NZ_CP129292
• Coordinates: 1133023..1133670 (+)
• Length: 215 a.a.
• NCBI ID: WP_301536143.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain KMGR2-43
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1128023..1138670

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QYH60_RS05995 (QYH60_05995)	-	1128152..1129330 (+)	1179	WP_012898345.1	SLC13 family permease	-
QYH60_RS06000 (QYH60_06000)	-	1129503..1130066 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
QYH60_RS06005 (QYH60_06005)	-	1130224..1130568 (+)	345	WP_259747958.1	hypothetical protein	-
QYH60_RS06010 (QYH60_06010)	-	1130673..1131605 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
QYH60_RS06015 (QYH60_06015)	-	1131602..1132963 (+)	1362	WP_039115830.1	ABC transporter permease	-
QYH60_RS06020 (QYH60_06020)	comEA	1133023..1133670 (+)	648	WP_301536143.1	ComEA family DNA-binding protein	Machinery gene
QYH60_RS06025 (QYH60_06025)	-	1133651..1135860 (+)	2210	Protein_1139	DNA internalization-related competence protein ComEC/Rec2	-
QYH60_RS06030 (QYH60_06030)	-	1136145..1136921 (+)	777	WP_058208158.1	alpha/beta hydrolase family protein	-
QYH60_RS06035 (QYH60_06035)	-	1137106..1137321 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
QYH60_RS06040 (QYH60_06040)	atpB	1137366..1138079 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
QYH60_RS06045 (QYH60_06045)	atpF	1138094..1138600 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23652.82 Da
• Isoelectric Point: 4.8659

>NTDB_id=852094 QYH60_RS06020 WP_301536143.1 1133023..1133670(+) (comEA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGVVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=852094 QYH60_RS06020 WP_301536143.1 1133023..1133670(+) (comEA) [Lactococcus lactis subsp. lactis strain KMGR2-43]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGTGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381