Detailed information NTG0085143

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FLY89_RS17770
• Genome accession: NZ_AP024415
• Coordinates: 3673035..3674093 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain NIPH17_0019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3668035..3679093

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLY89_RS17745 (KHAB170019_35400)	aroK	3668399..3668968 (-)	570	WP_000643712.1	shikimate kinase AroK	-
FLY89_RS17750	pilQ	3668980..3671051 (-)	2072	Protein_3484	type IV pilus secretin PilQ	-
FLY89_RS17755 (KHAB170019_35440)	pilP	3671119..3671646 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
FLY89_RS17760 (KHAB170019_35450)	pilO	3671657..3672397 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
FLY89_RS17765 (KHAB170019_35460)	pilN	3672394..3673035 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
FLY89_RS17770 (KHAB170019_35470)	pilM	3673035..3674093 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
FLY89_RS17775	ponA	3674256..3676758 (+)	2503	Protein_3489	penicillin-binding protein PBP1a	-
FLY89_RS17780 (KHAB170019_35500)	-	3676776..3677600 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
FLY89_RS17785 (KHAB170019_35510)	-	3677603..3677935 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=85143 FLY89_RS17770 WP_002014066.1 3673035..3674093(-) (pilM) [Acinetobacter baumannii strain NIPH17_0019]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=85143 FLY89_RS17770 WP_002014066.1 3673035..3674093(-) (pilM) [Acinetobacter baumannii strain NIPH17_0019]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCAGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423