Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JSX26_RS02775 Genome accession   NZ_AP024402
Coordinates   545675..546784 (-) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas caviae strain KAM376     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540675..551784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSX26_RS02750 (KAM376_05440) - 540809..541036 (+) 228 WP_049636474.1 hypothetical protein -
  JSX26_RS02755 (KAM376_05450) brnQ 541126..542433 (-) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -
  JSX26_RS02760 (KAM376_05460) - 542623..543333 (-) 711 WP_203764123.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  JSX26_RS02765 (KAM376_05470) srmB 543461..544681 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  JSX26_RS02770 (KAM376_05480) yaaA 544869..545642 (-) 774 WP_053288104.1 peroxide stress protein YaaA -
  JSX26_RS02775 (KAM376_05490) pilU 545675..546784 (-) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JSX26_RS02780 (KAM376_05500) pilT 546808..547842 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  JSX26_RS02785 (KAM376_05510) - 547882..548583 (+) 702 WP_203764125.1 YggS family pyridoxal phosphate-dependent enzyme -
  JSX26_RS02790 (KAM376_05520) proC 548706..549530 (+) 825 WP_039039961.1 pyrroline-5-carboxylate reductase -
  JSX26_RS02795 (KAM376_05530) - 549554..550105 (+) 552 WP_042014922.1 YggT family protein -
  JSX26_RS02800 (KAM376_05540) yggU 550105..550404 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  JSX26_RS02805 (KAM376_05550) - 550423..550839 (+) 417 WP_203764127.1 DUF4426 domain-containing protein -
  JSX26_RS02810 (KAM376_05560) - 550915..551745 (-) 831 WP_203764130.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=85036 JSX26_RS02775 WP_042882153.1 545675..546784(-) (pilU) [Aeromonas caviae strain KAM376]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=85036 JSX26_RS02775 WP_042882153.1 545675..546784(-) (pilU) [Aeromonas caviae strain KAM376]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAACGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTTCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment