Detailed information NTG0848334

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: QTV55_RS04455
• Genome accession: NZ_CP128523
• Coordinates: 865194..866594 (+)
• Length: 466 a.a.
• NCBI ID: WP_289365380.1
• Uniprot ID: -
• Organism: Mannheimia haemolytica strain 182812
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 860194..871594

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QTV55_RS04425	-	860268..861032 (-)	765	WP_006248395.1	TSUP family transporter	-
QTV55_RS04430	proB	861032..862123 (-)	1092	WP_006251346.1	glutamate 5-kinase	-
QTV55_RS04435	folA	862235..862729 (+)	495	WP_006248397.1	type 3 dihydrofolate reductase	-
QTV55_RS04440	-	862849..863079 (+)	231	WP_006248398.1	YgjV family protein	-
QTV55_RS04445	radA	863134..864513 (-)	1380	WP_040080753.1	DNA repair protein RadA	Machinery gene
QTV55_RS04450	pilA	864694..865173 (+)	480	WP_006248401.1	prepilin peptidase-dependent pilin	Machinery gene
QTV55_RS04455	pilB	865194..866594 (+)	1401	WP_289365380.1	GspE/PulE family protein	Machinery gene
QTV55_RS04460	pilC	866578..867774 (+)	1197	WP_006248403.1	type II secretion system F family protein	Machinery gene
QTV55_RS04465	-	867777..868433 (+)	657	WP_006248404.1	prepilin peptidase	-
QTV55_RS04470	coaE	868469..869104 (+)	636	WP_020831305.1	dephospho-CoA kinase	-
QTV55_RS04475	yacG	869086..869271 (+)	186	WP_006248406.1	DNA gyrase inhibitor YacG	-
QTV55_RS04480	holA	869272..870297 (-)	1026	WP_006253426.1	DNA polymerase III subunit delta	-
QTV55_RS04485	lptE	870340..870828 (-)	489	WP_006251337.1	LPS assembly lipoprotein LptE	-

Sequence

Protein

• Length: 466 a.a.
• Molecular weight: 52878.54 Da
• Isoelectric Point: 6.1046

>NTDB_id=848334 QTV55_RS04455 WP_289365380.1 865194..866594(+) (pilB) [Mannheimia haemolytica strain 182812]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDEL
KFLLNALSPEQSSRQSEIYEENTAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLIRLRIDGVLHIY
KTLPQSLSSRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRLQKNKPTQVDFLELGFNP
TQKANLRNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFL
RQDPDIIMLGEIRDEGSAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGGA
GCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide

• Length: 1401 bp

>NTDB_id=848334 QTV55_RS04455 WP_289365380.1 865194..866594(+) (pilB) [Mannheimia haemolytica strain 182812]
ATGGAGTATTCGGTAACGGATTTAAACAGCCAAAGGTTCATCGAGATTTCAGCAGAGCAGTGGCAGAAAAATTGCAATGA
AAAGCAGATTTTATTGCGATATTTAGCCATTCCGCTGCAAGAAACAGAAGAACAGCTTTGGCTAGGGATTGATGATATGC
AAAATCTCAATGCCTGTGAGATTTTTGCGTTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGATGAATTG
AAGTTTTTACTCAATGCCCTTTCGCCTGAGCAGAGTAGCCGGCAGAGTGAGATTTATGAGGAGAATACGGCGTATTCATT
GGCTCATTCGGAACAAGTGGATAGAAACGATCCGATTATCCAAATTTTAGAGAATTTATTTAAGTTCTGTTTGCAACACA
ACGCCTCAGATATTCATTTTGAACCACAAAAAGAGAAATTGCTTATTCGGCTGCGAATTGATGGCGTGCTACATATTTAT
AAAACGCTTCCGCAAAGTCTGTCTAGCCGATTAATTTCTCGCATTAAATTATTGGCAAAATTAGATATTAGCGAAACCCG
CTTGCCGCAAGATGGGCAGTTTCATTTTAAAACAATTTTAGCGGAAACCTTAGATTTCCGCGTTTCAACTTTACCTACCC
ATTTCGGTGAAAAAGTTGTTCTTCGGCTTCAAAAGAATAAGCCTACGCAGGTGGATTTTTTAGAATTGGGGTTTAACCCC
ACTCAGAAAGCCAATTTGCGGAATGCTCTGAGCCAACCTCAAGGGTTGATTTTAGTCACAGGCCCGACCGGGAGCGGTAA
AAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTAACTGCCGAAGACCCGATTGAAA
TTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAATTTAGATTTCAGTCAACTGTTAAGAACTTTTCTA
CGCCAAGATCCGGACATTATTATGCTTGGCGAAATTCGTGATGAAGGGAGTGCAGAAATGGCACTACGGGCGGCACAAAC
CGGGCATTTGGTGCTTTCAACTTTGCATACCAATGATGCCCCCTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAAAAG
AGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGGGCA
GGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCAAAAAATTTTGA
AAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATCAGG
CGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (29-92)

• Type II/IV secretion system protein (T2SSE) (131-392)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Glaesserella parasuis strain SC1401	72.017	98.927	0.712
pilB	Haemophilus influenzae Rd KW20	57.019	99.356	0.567
pilB	Haemophilus influenzae 86-028NP	56.061	99.142	0.556
pilB	Vibrio cholerae strain A1552	40.664	100	0.421
pilB	Vibrio campbellii strain DS40M4	40.541	100	0.418
pilB	Vibrio parahaemolyticus RIMD 2210633	38.833	100	0.414
pilB	Legionella pneumophila strain ERS1305867	39.344	100	0.412
pilB	Acinetobacter baumannii D1279779	36.401	100	0.382
pilB	Acinetobacter baylyi ADP1	36.475	100	0.382