Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QTV55_RS04450 Genome accession   NZ_CP128523
Coordinates   864694..865173 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 182812     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859694..870173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QTV55_RS04425 - 860268..861032 (-) 765 WP_006248395.1 TSUP family transporter -
  QTV55_RS04430 proB 861032..862123 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  QTV55_RS04435 folA 862235..862729 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  QTV55_RS04440 - 862849..863079 (+) 231 WP_006248398.1 YgjV family protein -
  QTV55_RS04445 radA 863134..864513 (-) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  QTV55_RS04450 pilA 864694..865173 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  QTV55_RS04455 pilB 865194..866594 (+) 1401 WP_289365380.1 GspE/PulE family protein Machinery gene
  QTV55_RS04460 pilC 866578..867774 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  QTV55_RS04465 - 867777..868433 (+) 657 WP_006248404.1 prepilin peptidase -
  QTV55_RS04470 coaE 868469..869104 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  QTV55_RS04475 yacG 869086..869271 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=848333 QTV55_RS04450 WP_006248401.1 864694..865173(+) (pilA) [Mannheimia haemolytica strain 182812]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=848333 QTV55_RS04450 WP_006248401.1 864694..865173(+) (pilA) [Mannheimia haemolytica strain 182812]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491