Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUC24_RS23970 Genome accession   NZ_CP128506
Coordinates   5180557..5182257 (+) Length   566 a.a.
NCBI ID   WP_096253228.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Fe11-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5175557..5187257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUC24_RS23950 (QUC24_23950) nadC 5177362..5178210 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUC24_RS23960 (QUC24_23960) - 5178391..5179704 (-) 1314 WP_289393647.1 O-antigen ligase family protein -
  QUC24_RS23965 (QUC24_23965) - 5179862..5180326 (-) 465 WP_023129290.1 pilin -
  QUC24_RS23970 (QUC24_23970) pilB 5180557..5182257 (+) 1701 WP_096253228.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUC24_RS23975 (QUC24_23975) pilC 5182261..5183478 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  QUC24_RS23980 (QUC24_23980) pilD 5183479..5184351 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QUC24_RS23985 (QUC24_23985) coaE 5184348..5184959 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  QUC24_RS23990 (QUC24_23990) yacG 5184956..5185156 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  QUC24_RS23995 (QUC24_23995) - 5185193..5185402 (-) 210 WP_003094660.1 hypothetical protein -
  QUC24_RS24000 (QUC24_24000) - 5185508..5186197 (-) 690 WP_196624286.1 energy-coupling factor ABC transporter permease -
  QUC24_RS24005 (QUC24_24005) - 5186194..5186664 (-) 471 WP_003094664.1 hypothetical protein -
  QUC24_RS24010 (QUC24_24010) - 5186661..5187086 (-) 426 WP_196624287.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62284.16 Da        Isoelectric Point: 5.4470

>NTDB_id=848094 QUC24_RS23970 WP_096253228.1 5180557..5182257(+) (pilB) [Pseudomonas aeruginosa strain Fe11-1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=848094 QUC24_RS23970 WP_096253228.1 5180557..5182257(+) (pilB) [Pseudomonas aeruginosa strain Fe11-1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTGAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGATTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.521

98.763

0.509
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

52.158

94.17

0.491
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378