Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V3Z63_RS10770 Genome accession   NZ_CP144289
Coordinates   2274248..2275756 (+) Length   502 a.a.
NCBI ID   WP_282502881.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CH1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2269248..2280756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3Z63_RS10750 (V3Z63_10750) - 2269278..2270489 (+) 1212 WP_012075064.1 O-succinylhomoserine sulfhydrylase -
  V3Z63_RS10755 (V3Z63_10755) - 2270486..2271253 (+) 768 WP_012075065.1 SDR family oxidoreductase -
  V3Z63_RS10760 (V3Z63_10760) xcpQ 2271340..2273316 (-) 1977 WP_012075066.1 GspD family T2SS secretin variant XcpQ -
  V3Z63_RS10765 (V3Z63_10765) xcpP 2273321..2274028 (-) 708 WP_049792030.1 type II secretion system protein N -
  V3Z63_RS10770 (V3Z63_10770) pilB 2274248..2275756 (+) 1509 WP_282502881.1 GspE family T2SS ATPase variant XcpR Machinery gene
  V3Z63_RS10775 (V3Z63_10775) xcpS 2275756..2276973 (+) 1218 WP_012075069.1 GspF family T2SS innner membrane protein variant XcpS -
  V3Z63_RS10780 (V3Z63_10780) xcpT 2276978..2277424 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  V3Z63_RS10785 (V3Z63_10785) xcpU 2277431..2277949 (+) 519 WP_012075070.1 GspH family T2SS minor pseudopilin variant XcpU -
  V3Z63_RS10790 (V3Z63_10790) xcpV 2277946..2278335 (+) 390 WP_003154139.1 GspI family T2SS minor pseudopilin variant XcpV -
  V3Z63_RS10795 (V3Z63_10795) xcpW 2278332..2279045 (+) 714 WP_012075071.1 GspJ family T2SS minor pseudopilin variant XcpW -
  V3Z63_RS10800 (V3Z63_10800) xcpX 2279042..2280043 (+) 1002 WP_012075072.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55380.55 Da        Isoelectric Point: 5.9434

>NTDB_id=846895 V3Z63_RS10770 WP_282502881.1 2274248..2275756(+) (pilB) [Pseudomonas aeruginosa strain CH1]
MMTAPLPDIAAPAAPPRRLPFSFAKGQGLLFLCLEEQYWLACRPQVEPAAIAEAQRFAGRRLPLKALGEEAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYLADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=846895 V3Z63_RS10770 WP_282502881.1 2274248..2275756(+) (pilB) [Pseudomonas aeruginosa strain CH1]
ATGATGACAGCCCCGTTACCCGATATCGCCGCGCCGGCCGCACCGCCACGGCGCCTGCCGTTCAGCTTCGCCAAGGGCCA
GGGCCTGCTGTTCCTGTGCCTGGAGGAACAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCCGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGTCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGAGGCTTTCAACCAGGCCCTGGCCGCGTCCTAC
CAGCACGATTCCTCCGCGGCCATGCAGCTCGCCGAAGACCTCGGCGGGAGTCTCGATCTCGCCGCCCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGATGCGCCGATCATCCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCCGACATCCACCTGGAGACCTTCGAGAAGCGCCTGGTGGTGCGCTTTCGCGTCGACGGGGTACTG
CGCGAGGTGCTCGAACCCAAGCGCGAGCTGGCGGCGTTGCTGGTATCGCGGATCAAGGTCATGGCCCGCCTGGACATCGC
CGAGAAGCGCATCCCCCAGGACGGCCGTATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGTGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGTGTGGTGCTGCGCCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAGACTGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGTTC
GGGCAAGACCACCACGCTCTATGCCAGCCTGACCACCCTCAACGACCGCACCCGCAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACTTTCGCCCGCGGTCTGCGCGCG
ATCCTCCGGCAGGACCCGGATGTGGTGATGGTCGGCGAGATCCGCGACCGCGAGACCGCCGAGATCGCCGTGCAGGCCTC
GCTGACCGGGCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAACCGTTCCTGCTGTCCTCCTCGATGCTCGGCGTGCTGGCCCAGCGCCTGGTACGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCTGGCGGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAATGCCACCAGCATGGCTACCGCGGGCGTACCGGGATCTATGAGCTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGCCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

43.737

93.825

0.41
  pilB/pilB1 Synechocystis sp. PCC 6803

41.837

97.61

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.294

84.661

0.4
  pilF Thermus thermophilus HB27

43.146

88.645

0.382
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilB Vibrio cholerae strain A1552

46.717

78.884

0.369
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.165

77.291

0.365