Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QSG84_RS09485 Genome accession   NZ_CP127893
Coordinates   2026638..2028344 (+) Length   568 a.a.
NCBI ID   WP_317032654.1    Uniprot ID   A0AA97F3C5
Organism   Acinetobacter sp. SAAs470     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2021638..2033344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSG84_RS09445 (QSG84_09440) nusA 2022400..2023884 (-) 1485 WP_317030167.1 transcription termination factor NusA -
  QSG84_RS09450 (QSG84_09445) rimP 2023916..2024440 (-) 525 WP_317030168.1 ribosome maturation factor RimP -
  QSG84_RS09475 (QSG84_09470) secG 2025237..2025566 (-) 330 WP_317030169.1 preprotein translocase subunit SecG -
  QSG84_RS09480 (QSG84_09475) tpiA 2025580..2026371 (-) 792 WP_317030170.1 triose-phosphate isomerase -
  QSG84_RS09485 (QSG84_09480) pilB 2026638..2028344 (+) 1707 WP_317032654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QSG84_RS09490 (QSG84_09485) pilC 2028375..2029601 (+) 1227 WP_317030171.1 type II secretion system F family protein Machinery gene
  QSG84_RS09495 (QSG84_09490) pilD 2029601..2030461 (+) 861 WP_317030172.1 A24 family peptidase Machinery gene
  QSG84_RS09500 (QSG84_09495) coaE 2030470..2031111 (+) 642 WP_317030173.1 dephospho-CoA kinase -
  QSG84_RS09505 (QSG84_09500) - 2031115..2032032 (-) 918 WP_317030174.1 DMT family transporter -
  QSG84_RS09510 (QSG84_09505) rlmB 2032070..2032822 (-) 753 WP_317030175.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QSG84_RS09515 (QSG84_09510) - 2033016..2033339 (-) 324 WP_317030176.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 64011.12 Da        Isoelectric Point: 6.6939

>NTDB_id=845127 QSG84_RS09485 WP_317032654.1 2026638..2028344(+) (pilB) [Acinetobacter sp. SAAs470]
MKLMHLQPKFTGFIQRLVDTGIVSTELMRNALESTRKNQQDIVAHLVQNHHISSYLIAQNIASEFGEPFFDLSVYDTRFL
VKANIDEKIIIKYNILPIFKRKNLLFIATANPIDLDALEAIRLNSKMHIETIIVEFDKLKWLIEQHYTGRAYVALNNDDT
AFDLHYEPIETTTNEETTYDESPIVKYINQLLLDAVHMGASDLHFEPYEKLYRVRYRIDGILRQIATPPLALANRLTSRL
KIMSQMDISEKRIPQDGRIKLKLSQQQSIDFRVNALPTLFGEKMVLRILDPSSAILGIDILGYEHDQKTLFINALKKPQG
MLLITGPTGSGKTVSLYTGLNIINQENINISTAEDPVEINLEGINQVNVNTKVGLSFSAALKSFLRQDPDIIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSINLVIAQRLVRKLCEQCKIPLDIPAASLFEMGFTA
TDLTRTDFQIYQANGCMACKEGYKGRIGLYEVMQITPNLSTLIMQDANALQIVQAAQHEGFYNLKRSGLIKVMQGLTSLQ
EINRVILE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=845127 QSG84_RS09485 WP_317032654.1 2026638..2028344(+) (pilB) [Acinetobacter sp. SAAs470]
ATTAAGTTGATGCATCTGCAACCTAAATTCACAGGCTTTATTCAGCGTCTGGTCGATACTGGTATCGTAAGTACTGAGCT
GATGCGTAATGCATTAGAAAGCACGAGAAAAAATCAACAAGATATTGTTGCTCATTTGGTACAAAACCATCATATCTCAT
CCTATTTGATTGCACAAAATATTGCCAGTGAGTTTGGTGAGCCTTTTTTTGATTTATCTGTTTACGATACCCGCTTTTTG
GTTAAAGCCAATATTGATGAAAAAATAATAATAAAATACAACATACTGCCAATCTTTAAACGTAAAAACTTACTTTTTAT
TGCTACGGCAAATCCAATTGATCTGGATGCTTTAGAAGCCATTCGTTTAAACAGTAAAATGCATATCGAAACCATTATTG
TCGAGTTTGATAAATTAAAATGGCTCATTGAACAACATTATACTGGTCGTGCTTATGTGGCATTAAACAATGATGACACT
GCATTTGACTTACACTATGAACCCATAGAAACAACAACAAATGAAGAAACGACGTATGATGAGTCACCTATTGTCAAATA
TATTAATCAATTACTATTAGATGCGGTTCATATGGGGGCATCTGATTTACATTTTGAACCTTACGAAAAACTTTATCGTG
TGCGTTATCGTATCGATGGTATTTTAAGGCAAATCGCAACCCCGCCTTTAGCACTGGCCAATCGCTTAACTTCACGTTTA
AAAATCATGTCACAAATGGATATTTCAGAAAAGCGCATTCCTCAAGATGGGCGAATCAAGCTAAAACTTTCTCAACAGCA
AAGTATTGATTTTCGTGTGAATGCTTTACCTACCTTATTTGGTGAAAAAATGGTATTGCGTATTCTAGATCCATCAAGTG
CCATACTGGGCATTGACATTTTAGGCTATGAACATGATCAAAAAACACTTTTTATCAATGCATTAAAAAAACCACAAGGG
ATGCTACTGATTACTGGTCCCACTGGCTCTGGTAAAACAGTTTCACTCTATACCGGCTTAAATATTATCAATCAAGAAAA
TATCAATATTTCTACTGCAGAAGATCCTGTAGAAATTAACCTCGAAGGTATTAATCAGGTCAATGTCAATACTAAAGTGG
GTCTAAGCTTTAGCGCCGCACTCAAATCTTTTTTACGTCAAGACCCAGATATTATTATGGTCGGTGAAATTCGTGATCTT
GAAACCGCCGAAATCGCCATCAAAGCAGCGCAAACAGGTCATATGGTCATGTCTACATTGCATACCAACAGTGCCGCTGA
AACACTGACACGTTTACGCAATATGGGTGTGGCAGCATTTAATATTGCCACATCGATTAACTTAGTCATTGCACAGCGCT
TGGTTCGTAAGCTTTGCGAACAATGTAAAATACCGCTTGATATTCCTGCTGCCAGTTTATTTGAAATGGGATTTACAGCA
ACGGATTTAACACGCACTGACTTTCAAATCTATCAAGCAAATGGATGCATGGCTTGCAAAGAAGGCTATAAAGGACGAAT
AGGTCTCTATGAAGTCATGCAAATTACACCTAATCTTTCTACACTGATTATGCAAGATGCTAATGCATTACAAATCGTAC
AAGCAGCGCAGCATGAAGGTTTTTATAATTTAAAACGCTCAGGATTAATTAAAGTCATGCAGGGACTCACCTCTTTACAA
GAAATCAATCGTGTCATTTTGGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

66.316

100

0.665
  pilB Acinetobacter baylyi ADP1

65.897

99.12

0.653
  pilB Legionella pneumophila strain ERS1305867

50.743

94.718

0.481
  pilB Vibrio campbellii strain DS40M4

48.307

98.768

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

50.189

93.31

0.468
  pilB Vibrio cholerae strain A1552

51.859

89.965

0.467
  pilF Neisseria gonorrhoeae MS11

47.207

97.711

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.944

100

0.382