Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QRO24_RS07695 Genome accession   NZ_CP127829
Coordinates   1600813..1602222 (-) Length   469 a.a.
NCBI ID   WP_018345911.1    Uniprot ID   -
Organism   Gallibacterium anatis strain OH/Ck/12965/20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1595813..1607222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRO24_RS07660 (QRO24_07615) - 1596097..1596756 (+) 660 WP_013746615.1 formate dehydrogenase subunit gamma -
  QRO24_RS07665 (QRO24_07620) fdhE 1596758..1597681 (+) 924 WP_039094142.1 formate dehydrogenase accessory protein FdhE -
  QRO24_RS07670 (QRO24_07625) - 1597747..1598019 (-) 273 WP_039133889.1 GNAT family N-acetyltransferase -
  QRO24_RS07675 (QRO24_07630) yacG 1598105..1598323 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  QRO24_RS07680 (QRO24_07635) coaE 1598313..1598945 (-) 633 WP_409025545.1 dephospho-CoA kinase -
  QRO24_RS07685 (QRO24_07640) - 1598960..1599592 (-) 633 WP_409025546.1 prepilin peptidase -
  QRO24_RS07690 (QRO24_07645) - 1599589..1600797 (-) 1209 WP_335793447.1 type II secretion system F family protein -
  QRO24_RS07695 (QRO24_07650) pilB 1600813..1602222 (-) 1410 WP_018345911.1 GspE/PulE family protein Machinery gene
  QRO24_RS07700 (QRO24_07655) pilA 1602239..1602700 (-) 462 WP_335793446.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QRO24_RS07705 (QRO24_07660) ampD 1602805..1603359 (+) 555 WP_264779690.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QRO24_RS07710 (QRO24_07665) - 1603446..1603781 (+) 336 WP_013746603.1 hypothetical protein -
  QRO24_RS07715 (QRO24_07670) - 1603890..1605284 (+) 1395 WP_335793445.1 chloride channel protein -
  QRO24_RS07720 (QRO24_07675) dusA 1605265..1606245 (+) 981 WP_039092431.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52818.20 Da        Isoelectric Point: 5.1780

>NTDB_id=844372 QRO24_RS07695 WP_018345911.1 1600813..1602222(-) (pilB) [Gallibacterium anatis strain OH/Ck/12965/20]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQQITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=844372 QRO24_RS07695 WP_018345911.1 1600813..1602222(-) (pilB) [Gallibacterium anatis strain OH/Ck/12965/20]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTAGCTGTACCGTTGCAGGAACAAGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCCGCTTGCGAAACCTTTGCTTTTCTTTCCGGCAAACAGATTGAGCCTGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATCGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTTCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACGG
CGGAAGATCCGATAGAACTGCAATTAAACGGTATTATTCAGACCCAAGCAAATGCCGCAATCGGGTTAACTTTTGCGACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGACATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AACAGTTGGGAATTGCACAACACGAATTGGAAAATTCATTATTGCTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGACGGTTATCGCGGACGAACCGGCGTTTATCAACTGCT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTTAAATTACAGCAACAGATCA
CGACAACAGCAGAGGTTGAGCGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.43

97.228

0.578
  pilB Haemophilus influenzae Rd KW20

59.43

97.228

0.578
  pilB Glaesserella parasuis strain SC1401

53.846

99.787

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

38.878

100

0.414
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB Acinetobacter baumannii D1279779

37.832

100

0.394
  pilB Legionella pneumophila strain ERS1305867

36.157

100

0.373
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373