Detailed information NTG0844258

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: P9963_RS29935
• Genome accession: NZ_CP143649
• Coordinates: 6399981..6401045 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain CPO506
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6394981..6406045

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P9963_RS29910 (P9963_029910)	aroK	6395516..6396034 (-)	519	WP_003095833.1	shikimate kinase AroK	-
P9963_RS29915 (P9963_029915)	pilQ	6396046..6398190 (-)	2145	WP_285239537.1	type 4a pilus secretin PilQ	Machinery gene
P9963_RS29920 (P9963_029920)	comL/pilP	6398244..6398768 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
P9963_RS29925 (P9963_029925)	pilO	6398765..6399388 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
P9963_RS29930 (P9963_029930)	pilN	6399385..6399981 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
P9963_RS29935 (P9963_029935)	pilM	6399981..6401045 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
P9963_RS29940 (P9963_029940)	-	6401230..6403698 (+)	2469	WP_025297613.1	penicillin-binding protein 1A	-
P9963_RS29945 (P9963_029945)	-	6403803..6405071 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=844258 P9963_RS29935 WP_003110888.1 6399981..6401045(-) (pilM) [Pseudomonas aeruginosa strain CPO506]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=844258 P9963_RS29935 WP_003110888.1 6399981..6401045(-) (pilM) [Pseudomonas aeruginosa strain CPO506]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTCCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466