Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P9935_RS29915 Genome accession   NZ_CP143648
Coordinates   6399037..6399609 (-) Length   190 a.a.
NCBI ID   WP_121410843.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CPO459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6394037..6404609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9935_RS29895 (P9935_029895) aroB 6394632..6395738 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  P9935_RS29900 (P9935_029900) aroK 6395788..6396306 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P9935_RS29905 (P9935_029905) pilQ 6396318..6398462 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  P9935_RS29910 (P9935_029910) comL/pilP 6398516..6399040 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P9935_RS29915 (P9935_029915) pilO 6399037..6399609 (-) 573 WP_121410843.1 type 4a pilus biogenesis protein PilO Machinery gene
  P9935_RS29920 (P9935_029920) pilN 6399606..6400202 (-) 597 WP_015647905.1 type 4a pilus biogenesis protein PilN Machinery gene
  P9935_RS29925 (P9935_029925) pilM 6400202..6401266 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  P9935_RS29930 (P9935_029930) - 6401451..6403919 (+) 2469 WP_025297613.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 190 a.a.        Molecular weight: 20881.13 Da        Isoelectric Point: 4.8510

>NTDB_id=844148 P9935_RS29915 WP_121410843.1 6399037..6399609(-) (pilO) [Pseudomonas aeruginosa strain CPO459]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAANLEAYKAQMKEMEESFG
ALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATFVSGVSSLPRIVTLHDFE
IKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 573 bp        

>NTDB_id=844148 P9935_RS29915 WP_121410843.1 6399037..6399609(-) (pilO) [Pseudomonas aeruginosa strain CPO459]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGAACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGC
GCCTTGCTGCGGCAGTTGCCCAGCGACACCGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGG
CCTGGAGTTCGAGGAAATCAAGCTGCTTCCCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGG
TCGGCGGCTACCACGACCTGGCGACCTTCGTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAG
ATCAAGCCGGTCGCGCCCGGCAGCACGTCCAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGG
GCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(53-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

43.005

100

0.437
  pilO Acinetobacter baumannii D1279779

42.932

100

0.432
  comO/pilO Acinetobacter baylyi ADP1

42.932

100

0.432