Detailed information NTG0843416

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QRA07_RS15200
• Genome accession: NZ_CP127321
• Coordinates: 3204864..3205982 (+)
• Length: 372 a.a.
• NCBI ID: WP_005070855.1
• Uniprot ID: A0A013SVN8
• Organism: Acinetobacter pittii strain PC870
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3199864..3210982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRA07_RS15190 (QRA07_15230)	yggS	3202988..3203680 (-)	693	WP_130121013.1	YggS family pyridoxal phosphate-dependent enzyme	-
QRA07_RS15195 (QRA07_15235)	pilT	3203807..3204844 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
QRA07_RS15200 (QRA07_15240)	pilU	3204864..3205982 (+)	1119	WP_005070855.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QRA07_RS15205 (QRA07_15245)	fur	3206052..3206489 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
QRA07_RS15210 (QRA07_15250)	omlA	3206601..3206999 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
QRA07_RS15215 (QRA07_15255)	-	3207064..3207378 (-)	315	WP_002121714.1	RnfH family protein	-
QRA07_RS15220 (QRA07_15260)	-	3207378..3208445 (-)	1068	WP_005075306.1	hypothetical protein	-
QRA07_RS15225 (QRA07_15265)	-	3208599..3209054 (-)	456	WP_002121698.1	bacteriohemerythrin	-
QRA07_RS15230 (QRA07_15270)	-	3209306..3210154 (-)	849	WP_057062207.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41931.15 Da
• Isoelectric Point: 6.6404

>NTDB_id=843416 QRA07_RS15200 WP_005070855.1 3204864..3205982(+) (pilU) [Acinetobacter pittii strain PC870]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=843416 QRA07_RS15200 WP_005070855.1 3204864..3205982(+) (pilU) [Acinetobacter pittii strain PC870]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTAAGGCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTGCTTAAAGATTTATCAATGACCAAACGCGGTATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTTGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCCGCAATCGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCGGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A013SVN8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363