Detailed information NTG0843074

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N024_RS13820
• Genome accession: NZ_CP127246
• Coordinates: 3265176..3266345 (-)
• Length: 389 a.a.
• NCBI ID: WP_024663648.1
• Uniprot ID: -
• Organism: Pseudomonas syringae CC457
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3260176..3271345

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N024_RS13805 (N024_13805)	-	3261463..3262524 (+)	1062	WP_024663646.1	microcin C ABC transporter permease YejB	-
N024_RS13810 (N024_13810)	-	3262524..3263546 (+)	1023	WP_024663647.1	ABC transporter permease	-
N024_RS13815 (N024_13815)	-	3263548..3265125 (+)	1578	WP_003435513.1	ABC transporter ATP-binding protein	-
N024_RS13820 (N024_13820)	pilU	3265176..3266345 (-)	1170	WP_024663648.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N024_RS13825 (N024_13825)	-	3266469..3266744 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
N024_RS13830 (N024_13830)	pgm	3266819..3268465 (-)	1647	WP_024663649.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
N024_RS13835 (N024_13835)	-	3268629..3270956 (-)	2328	WP_024663650.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42623.67 Da
• Isoelectric Point: 5.8676

>NTDB_id=843074 N024_RS13820 WP_024663648.1 3265176..3266345(-) (pilU) [Pseudomonas syringae CC457]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTAPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=843074 N024_RS13820 WP_024663648.1 3265176..3266345(-) (pilU) [Pseudomonas syringae CC457]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTACCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGAGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCCGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTACTGCTTGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACCGGTT
CGGGCAAGTCGACCTCGTTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGTCGTGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGTGAAGAAGAGGCCCTCAAGAACGCCGACTCACAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGTGAAGGCGGCGCCGGAACACTGACCACAGCGCCGCCAGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGAGCCGGGTCCGCAAGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.331	99.486	0.56
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	50.829	93.059	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.153	97.172	0.38
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362