Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N024_RS04300 Genome accession   NZ_CP127246
Coordinates   945572..947266 (-) Length   564 a.a.
NCBI ID   WP_024663009.1    Uniprot ID   A0A6B2AV38
Organism   Pseudomonas syringae CC457     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 940572..952266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N024_RS04265 (N024_04265) - 940779..941312 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  N024_RS04270 (N024_04270) - 941309..941938 (-) 630 WP_003406555.1 DUF1780 domain-containing protein -
  N024_RS04275 (N024_04275) - 942064..942525 (+) 462 WP_024663005.1 hypothetical protein -
  N024_RS04280 (N024_04280) yacG 942652..942861 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N024_RS04285 (N024_04285) coaE 942858..943481 (-) 624 WP_024663006.1 dephospho-CoA kinase -
  N024_RS04290 (N024_04290) pilD 943478..944350 (-) 873 WP_024663007.1 A24 family peptidase Machinery gene
  N024_RS04295 (N024_04295) pilC 944352..945569 (-) 1218 WP_024663008.1 type II secretion system F family protein Machinery gene
  N024_RS04300 (N024_04300) pilB 945572..947266 (-) 1695 WP_024663009.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N024_RS04305 (N024_04305) - 947494..947916 (+) 423 WP_024663010.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N024_RS04310 (N024_04310) - 948011..948556 (-) 546 WP_004415097.1 DUF1523 family protein -
  N024_RS04315 (N024_04315) - 948676..949674 (+) 999 WP_024663011.1 ATP-grasp domain-containing protein -
  N024_RS04320 (N024_04320) - 949729..950868 (+) 1140 WP_024663012.1 trypsin-like peptidase domain-containing protein -
  N024_RS04325 (N024_04325) - 950935..952068 (+) 1134 WP_003392690.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62408.72 Da        Isoelectric Point: 6.2545

>NTDB_id=843062 N024_RS04300 WP_024663009.1 945572..947266(-) (pilB) [Pseudomonas syringae CC457]
MTDAVLTGLAKQLVLAELLTESVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISKQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=843062 N024_RS04300 WP_024663009.1 945572..947266(-) (pilB) [Pseudomonas syringae CC457]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAACAATTGGTCCTAGCCGAGCTTCTTACTGAATCTGTTGCGCAACAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAATTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGACCAGTTTGGCGTTCCGTTCATGGACCTCGCCAGCCTTGACAAGGAAAGCCAGCCCAAAGGCTTG
GTCAGTGAAAAGCTGGTTCGCCAGCATCACGCACTGCCGCTGTGGCGTCGCGGTAACAAGCTGTTTATCGGGATTTCGGA
CCCGACCAATCATCAGGCAGTCACTGATATTCAGTTCAGCACTGGCCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACTGTTGCGATAGACCGGTTTTTTGACAGCGACAGCGGGCTGGGCAATCTGGAAGATGTCGATCTGGGCCTGGAT
ATCGAACCGGCCGATGGCAAGGAAACCACGCTAGCTACACAGAATGACGCTGACGATGCCCCGGTGGTGCGCTTCGTCAA
CAAGATGCTTATGGATGCCATCCGACTAGGATCATCCGACTTGCACTTCGAGCCCTATGAAAAGATCTTCCGCGTACGTT
TGCGTACCGATGGCATCCTGCACGAAGTCGCCAGACCGCCGATCCATTTGGCAAACCGTATTGCTGCGCGTCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCCTGCCCACATTGTGGGGCGAGAAGATCGTAATGCGGATTCTCGACCCAACCAGTGCGCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCTGATCAAAAGGCACTGTACTTAGAGGCGCTTAAACTGCCGCAAGGGATGATT
CTTGTGACTGGCCCTACAGGTTCAGGCAAGACTGTCTCTCTCTATACCGGCCTGAACATTCTAAACACTGTGGACATTAA
CATTTCCACAGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGCCAAGGACTGG
ACTTTTCCCAAGCCTTGCGCGCGTTTTTGCGTCAAGATCCCGACGTGATTATGGTCGGCGAAATACGAGATCTGGAAACT
GCCGAAATCGCCATCAAAGCCTCGCAGACCGGCCATATGGTGCTTTCCACATTGCACACCAACAGCGCTGCGGAAACGCT
GACCCGCCTGCATCACATGGGCGTTGCAGCCTTCAACATTGCTACAGCGATCAACCTGATTATTGCGCAGCGACTGGCAC
GTAAGCTGTGTAGCCACTGCAAAAAAGAACTGGATATTCCTCGCGAGACACTGATTCAGGAAGGCTTTCCAGAAGCCAAA
ATCGGCACTTTTAAGGTATACGGCCCGGTGGGCTGTGAGCACTGCAACGGGGGTTACCGAGGCAGGGTGGGCATTTACGA
AGTGGTCAAGAAAACACCGGAGCTGGAGCGCATCATCATGGAAGAGGGTAACTCACTGGAGATTTCCAAGCAGATGCGCA
AGGACGGTTTCAACGACCTGCGAACCTCGGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B2AV38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.201

100

0.585
  pilB Acinetobacter baylyi ADP1

56.36

100

0.566
  pilB Legionella pneumophila strain ERS1305867

55.536

100

0.56
  pilB Vibrio parahaemolyticus RIMD 2210633

50.984

99.113

0.505
  pilB Vibrio cholerae strain A1552

50.534

99.645

0.504
  pilB Vibrio campbellii strain DS40M4

49.645

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

38.869

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

93.44

0.367