Detailed information NTG0843060

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N024_RS04290
• Genome accession: NZ_CP127246
• Coordinates: 943478..944350 (-)
• Length: 290 a.a.
• NCBI ID: WP_024663007.1
• Uniprot ID: A0A6B2AZR3
• Organism: Pseudomonas syringae CC457
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 938478..949350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N024_RS04260 (N024_04260)	-	939264..940562 (-)	1299	WP_024663004.1	NAD(P)/FAD-dependent oxidoreductase	-
N024_RS04265 (N024_04265)	-	940779..941312 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
N024_RS04270 (N024_04270)	-	941309..941938 (-)	630	WP_003406555.1	DUF1780 domain-containing protein	-
N024_RS04275 (N024_04275)	-	942064..942525 (+)	462	WP_024663005.1	hypothetical protein	-
N024_RS04280 (N024_04280)	yacG	942652..942861 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
N024_RS04285 (N024_04285)	coaE	942858..943481 (-)	624	WP_024663006.1	dephospho-CoA kinase	-
N024_RS04290 (N024_04290)	pilD	943478..944350 (-)	873	WP_024663007.1	A24 family peptidase	Machinery gene
N024_RS04295 (N024_04295)	pilC	944352..945569 (-)	1218	WP_024663008.1	type II secretion system F family protein	Machinery gene
N024_RS04300 (N024_04300)	pilB	945572..947266 (-)	1695	WP_024663009.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N024_RS04305 (N024_04305)	-	947494..947916 (+)	423	WP_024663010.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
N024_RS04310 (N024_04310)	-	948011..948556 (-)	546	WP_004415097.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31995.22 Da
• Isoelectric Point: 8.2910

>NTDB_id=843060 N024_RS04290 WP_024663007.1 943478..944350(-) (pilD) [Pseudomonas syringae CC457]
MPLLDLLACSPLAFVTTCCILGLIIGSFLNVVVYRIPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=843060 N024_RS04290 WP_024663007.1 943478..944350(-) (pilD) [Pseudomonas syringae CC457]
ATGCCCCTCCTCGACCTCCTGGCCTGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCATCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCATGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTATCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCATACGTGGCCTGGCATTTCGGGTTCGGCTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTCACATCGCTGAACGATGCGCTTTGGGGCGCGGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTTACAGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A6B2AZR3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476