Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VP464_RS00175 Genome accession   NZ_CP142892
Coordinates   10943..12433 (-) Length   496 a.a.
NCBI ID   WP_001166319.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain B20AB01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5943..17433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP464_RS00145 (VP464_00145) gigA 6936..7904 (+) 969 WP_000273589.1 RsbU family protein phosphatase GigA -
  VP464_RS00150 (VP464_00150) gigB 8007..8528 (+) 522 WP_000103064.1 anti-anti-sigma factor GigB -
  VP464_RS00155 (VP464_00155) - 8555..9379 (+) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  VP464_RS00160 (VP464_00160) - 9394..9771 (+) 378 WP_000266123.1 DUF4951 domain-containing protein -
  VP464_RS00165 (VP464_00165) - 9932..10369 (+) 438 WP_000371729.1 MarR family winged helix-turn-helix transcriptional regulator -
  VP464_RS00170 (VP464_00170) - 10457..10888 (+) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  VP464_RS00175 (VP464_00175) pilB 10943..12433 (-) 1491 WP_001166319.1 type II secretion system ATPase GspE Machinery gene
  VP464_RS00180 (VP464_00180) - 12530..13351 (+) 822 WP_000462729.1 hypothetical protein -
  VP464_RS00185 (VP464_00185) - 13361..14278 (+) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  VP464_RS00190 (VP464_00190) - 14268..17075 (-) 2808 WP_000276857.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55389.32 Da        Isoelectric Point: 6.2037

>NTDB_id=842284 VP464_RS00175 WP_001166319.1 10943..12433(-) (pilB) [Acinetobacter baumannii strain B20AB01]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHIFHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=842284 VP464_RS00175 WP_001166319.1 10943..12433(-) (pilB) [Acinetobacter baumannii strain B20AB01]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTA
GCTGCAGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCTGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAACACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTAGATATGACTTTTGCTCGAGCTTTAAAAGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTAGAAACTGCTGAAATTGCGGTGCAGGCATCTTTAACAGGTCATTTGGTGTTATCAACACT
ACATACCAATACCGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTTCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

42.008

98.387

0.413
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio cholerae strain A1552

48.13

80.847

0.389
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

39.917

96.976

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.264

81.048

0.383
  pilB Acinetobacter baylyi ADP1

47.315

78.831

0.373