Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VMR32_RS09435 Genome accession   NZ_CP142601
Coordinates   2011009..2012517 (+) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain Tongji     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2006009..2017517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VMR32_RS09415 (VMR32_09415) - 2006039..2007250 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  VMR32_RS09420 (VMR32_09420) - 2007247..2008014 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  VMR32_RS09425 (VMR32_09425) xcpQ 2008101..2010077 (-) 1977 WP_034015051.1 GspD family T2SS secretin variant XcpQ -
  VMR32_RS09430 (VMR32_09430) xcpP 2010082..2010789 (-) 708 WP_003119750.1 type II secretion system protein N -
  VMR32_RS09435 (VMR32_09435) pilB 2011009..2012517 (+) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  VMR32_RS09440 (VMR32_09440) xcpS 2012517..2013734 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  VMR32_RS09445 (VMR32_09445) xcpT 2013739..2014185 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  VMR32_RS09450 (VMR32_09450) xcpU 2014192..2014710 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  VMR32_RS09455 (VMR32_09455) xcpV 2014707..2015096 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  VMR32_RS09460 (VMR32_09460) xcpW 2015093..2015806 (+) 714 WP_003110067.1 GspJ family T2SS minor pseudopilin variant XcpW -
  VMR32_RS09465 (VMR32_09465) xcpX 2015803..2016804 (+) 1002 WP_016852649.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=841463 VMR32_RS09435 WP_003091377.1 2011009..2012517(+) (pilB) [Pseudomonas aeruginosa strain Tongji]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=841463 VMR32_RS09435 WP_003091377.1 2011009..2012517(+) (pilB) [Pseudomonas aeruginosa strain Tongji]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCACGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCAAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367