Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QP019_RS07735 Genome accession   NZ_CP126978
Coordinates   1568115..1569524 (-) Length   469 a.a.
NCBI ID   WP_285095953.1    Uniprot ID   -
Organism   Gallibacterium anatis strain YJ922     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1563115..1574524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP019_RS07700 (QP019_07700) - 1563723..1564859 (-) 1137 WP_285095947.1 transposase -
  QP019_RS07705 (QP019_07705) - 1564891..1565022 (-) 132 WP_285095948.1 hypothetical protein -
  QP019_RS07710 (QP019_07710) - 1565053..1565325 (-) 273 WP_039084192.1 GNAT family N-acetyltransferase -
  QP019_RS07715 (QP019_07715) yacG 1565407..1565625 (-) 219 WP_285095949.1 DNA gyrase inhibitor YacG -
  QP019_RS07720 (QP019_07720) coaE 1565615..1566247 (-) 633 WP_285095950.1 dephospho-CoA kinase -
  QP019_RS07725 (QP019_07725) - 1566262..1566894 (-) 633 WP_230589045.1 prepilin peptidase -
  QP019_RS07730 (QP019_07730) - 1566891..1568099 (-) 1209 WP_285095951.1 type II secretion system F family protein -
  QP019_RS07735 (QP019_07735) pilB 1568115..1569524 (-) 1410 WP_285095953.1 GspE/PulE family protein Machinery gene
  QP019_RS07740 (QP019_07740) pilA 1569541..1570002 (-) 462 WP_039148357.1 prepilin peptidase-dependent pilin Machinery gene
  QP019_RS07745 (QP019_07745) ampD 1570107..1570661 (+) 555 WP_285095954.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QP019_RS07750 (QP019_07750) - 1570748..1571083 (+) 336 WP_013746603.1 hypothetical protein -
  QP019_RS07755 (QP019_07755) - 1571192..1572586 (+) 1395 WP_094933117.1 chloride channel protein -
  QP019_RS07760 (QP019_07760) dusA 1572567..1573547 (+) 981 WP_285095955.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52876.29 Da        Isoelectric Point: 5.3117

>NTDB_id=840983 QP019_RS07735 WP_285095953.1 1568115..1569524(-) (pilB) [Gallibacterium anatis strain YJ922]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAISLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=840983 QP019_RS07735 WP_285095953.1 1568115..1569524(-) (pilB) [Gallibacterium anatis strain YJ922]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTGGCGGTACCGTTGCAGGAACAGGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCCGCTTGCGAAACCTTTGCTTTTCTTTCCGGCAAACAGATTGAGCCTGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATCGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGACTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTTCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACAG
CGGAAGATCCGATAGAACTGCAATTAAACGGCATTATTCAGACCCAAGCAAATGCCGCAATCAGTTTAACTTTTGCGACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTGATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCAGCACAGACAGGGCATTTAGTGTTATCAACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AGCAGTTGGGAATTGCACAACACGAATTGGAAAATTCATTATTGCTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGACGGTTATCGCGGACGAACCGGCGTTTATCAACTGCT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTGAAATTACAGCAACGGATCA
CGACAACAGCAGAGGTTGAGCGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.211

97.228

0.576
  pilB Haemophilus influenzae Rd KW20

59.211

97.228

0.576
  pilB Glaesserella parasuis strain SC1401

53.632

99.787

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

38.677

100

0.412
  pilB Vibrio cholerae strain A1552

39.627

100

0.407
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.405
  pilB Acinetobacter baylyi ADP1

39.278

94.456

0.371
  pilB Legionella pneumophila strain ERS1305867

35.95

100

0.371
  pilB Acinetobacter baumannii D1279779

38.902

93.177

0.362